caa a22 4500 605502331 CHVBK 20210128100605.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00253-015-6922-5 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6922-5 The ZntA-like NpunR4017 plays a key role in maintaining homeostatic levels of zinc in Nostoc punctiforme [Elektronische Daten] [L. Hudek, L. Bräu, A. Michalczyk, B. Neilan, J. Meeks, M. Ackland] Analysis of cellular response to zinc exposure provides insights into how organisms maintain homeostatic levels of zinc that are essential, while avoiding potentially toxic cytosolic levels. Using the cyanobacterium Nostoc punctiforme as a model, qRT-PCR analyses established a profile of the changes in relative mRNA levels of the ZntA-like zinc efflux transporter NpunR4017 in response to extracellular zinc. In cells treated with 18μM of zinc for 1h, NpunR4017 mRNA levels increased by up to 1300% above basal levels. The accumulation and retention of radiolabelled 65Zn by NpunR4107-deficient and overexpressing strains were compared to wild-type levels. Disruption of NpunR4017 resulted in a significant increase in zinc accumulation up to 24% greater than the wild type, while cells overexpressing NpunR4107 accumulated 22% less than the wild type. Accumulation of 65Zn in ZntA− Escherichia coli overexpressing NpunR4017 was reduced by up to 21%, indicating the capacity for NpunR4017 to compensate for the loss of ZntA. These findings establish the newly identified NpunR4017 as a zinc efflux transporter and a key transporter for maintaining zinc homeostasis in N. punctiforme. Springer-Verlag Berlin Heidelberg, 2015 Zinc nationallicence Cyanobacteria nationallicence ZntA nationallicence P-type ATPase nationallicence Metal efflux nationallicence Zinc homeostasis nationallicence Hudek L. Centre for Regional and Rural Futures, Deakin University, 221 Burwood Hwy, 3125, Burwood, Victoria, Australia aut Bräu L. Centre for Regional and Rural Futures, Deakin University, 221 Burwood Hwy, 3125, Burwood, Victoria, Australia aut Michalczyk A. Centre for Cellular and Molecular Biology, School of Life and Environmental Sciences, Deakin University, 221 Burwood Hwy, 3125, Burwood, Victoria, Australia aut Neilan B. Australian Centre for Astrobiology and School of Biotechnology and Biological Sciences, University of New South Wales, 2052, Sydney, NSW, Australia aut Meeks J. Department of Microbiology, University of California, 95616, Davis, CA, USA aut Ackland M. Centre for Cellular and Molecular Biology, School of Life and Environmental Sciences, Deakin University, 221 Burwood Hwy, 3125, Burwood, Victoria, Australia aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10559-10574 0175-7598 99:24<10559 2015 99 253 https://doi.org/10.1007/s00253-015-6922-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6922-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hudek L. Centre for Regional and Rural Futures, Deakin University, 221 Burwood Hwy, 3125, Burwood, Victoria, Australia aut NATIONALLICENCE 700 1- Bräu L. Centre for Regional and Rural Futures, Deakin University, 221 Burwood Hwy, 3125, Burwood, Victoria, Australia aut NATIONALLICENCE 700 1- Michalczyk A. Centre for Cellular and Molecular Biology, School of Life and Environmental Sciences, Deakin University, 221 Burwood Hwy, 3125, Burwood, Victoria, Australia aut NATIONALLICENCE 700 1- Neilan B. Australian Centre for Astrobiology and School of Biotechnology and Biological Sciences, University of New South Wales, 2052, Sydney, NSW, Australia aut NATIONALLICENCE 700 1- Meeks J. Department of Microbiology, University of California, 95616, Davis, CA, USA aut NATIONALLICENCE 700 1- Ackland M. Centre for Cellular and Molecular Biology, School of Life and Environmental Sciences, Deakin University, 221 Burwood Hwy, 3125, Burwood, Victoria, Australia aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10559-10574 0175-7598 99:24<10559 2015 99 253