Capsular polysaccharide production by Streptococcus pneumoniae serotype 1: from strain selection to fed-batch cultivation
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6928-z |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6928-z | ||
| 245 | 0 | 0 | |a Capsular polysaccharide production by Streptococcus pneumoniae serotype 1: from strain selection to fed-batch cultivation |h [Elektronische Daten] |c [Bruno Marthos, Anne Ferri, Douglas de Figueiredo, Teresa Zangirolami, Viviane Gonçalves] |
| 520 | 3 | |a Streptococcus pneumoniae is a human pathogen largely transmitted by aerosols. Vaccines are the main strategy against this pathogen, and the capsular polysaccharide (PS) is its major antigen. S. pneumoniae serotype 1 is associated with large outbreaks and epidemics of invasive diseases. The aims of this work were to screen serotype 1 strains to identify the best PS1 producer, evaluate three peptones for PS1 production, investigate the effects of culture medium components using a design of experiments (DoE), a statistic tool for optimization, and propose a new medium/cultivation strategy. After flask cultivation of nine strains, two that produced high PS1 and biomass values were chosen for further evaluation in the bioreactor, and ST595/01 was chosen as the best PS1 producer strain. Among the peptones tested (Casamino acids, Soytone, and Phytone), the highest PS1 production (298mg/L) was reached with Phytone. Next, DoE (24-1) was performed to evaluate the effects of yeast extract (YE), Phytone, L-asparagine (Asn), and L-glutamine (Gln), yielding the following results: Phytone presented positive effects (p < 0.05) for maximum production of biomass, PS1, acetate, and lactate; YE showed positive effects for biomass and acid production (p < 0.05); Gln exerted a minor positive effect on PS1 yield factor on glucose (p < 0.1); and Asn presented only an effect on acetate production (p < 0.1). Hence, a new culture medium was formulated based on Phytone, YE, and glucose, and batch and fed-batch cultivations were evaluated. The fed-batch cultivation showed almost 2 times the biomass and 2.5 times the PS1 production as the batch culture, and 8-10 times higher PS1 production than has been previously reported. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Pneumococcus |2 nationallicence | |
| 690 | 7 | |a Capsular polysaccharide production |2 nationallicence | |
| 690 | 7 | |a Strain screening |2 nationallicence | |
| 690 | 7 | |a Peptone selection |2 nationallicence | |
| 690 | 7 | |a Design of experiments |2 nationallicence | |
| 690 | 7 | |a Fed-batch cultivation |2 nationallicence | |
| 700 | 1 | |a Marthos |D Bruno |u Laboratório de Bioprocessos, Centro de Biotecnologia, Instituto Butantan, Av. Vital Brasil 1500, 05503-900, São Paulo, SP, Brazil |4 aut | |
| 700 | 1 | |a Ferri |D Anne |u Laboratório de Bioprocessos, Centro de Biotecnologia, Instituto Butantan, Av. Vital Brasil 1500, 05503-900, São Paulo, SP, Brazil |4 aut | |
| 700 | 1 | |a de Figueiredo |D Douglas |u Laboratório de Bioprocessos, Centro de Biotecnologia, Instituto Butantan, Av. Vital Brasil 1500, 05503-900, São Paulo, SP, Brazil |4 aut | |
| 700 | 1 | |a Zangirolami |D Teresa |u Departamento de Engenharia Química, Universidade Federal de São Carlos, Rodovia Washington Luís km 235, 13565-905, São Carlos, SP, Brazil |4 aut | |
| 700 | 1 | |a Gonçalves |D Viviane |u Laboratório de Bioprocessos, Centro de Biotecnologia, Instituto Butantan, Av. Vital Brasil 1500, 05503-900, São Paulo, SP, Brazil |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/24(2015-12-01), 10447-10456 |x 0175-7598 |q 99:24<10447 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6928-z |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6928-z |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Marthos |D Bruno |u Laboratório de Bioprocessos, Centro de Biotecnologia, Instituto Butantan, Av. Vital Brasil 1500, 05503-900, São Paulo, SP, Brazil |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ferri |D Anne |u Laboratório de Bioprocessos, Centro de Biotecnologia, Instituto Butantan, Av. Vital Brasil 1500, 05503-900, São Paulo, SP, Brazil |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a de Figueiredo |D Douglas |u Laboratório de Bioprocessos, Centro de Biotecnologia, Instituto Butantan, Av. Vital Brasil 1500, 05503-900, São Paulo, SP, Brazil |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zangirolami |D Teresa |u Departamento de Engenharia Química, Universidade Federal de São Carlos, Rodovia Washington Luís km 235, 13565-905, São Carlos, SP, Brazil |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Gonçalves |D Viviane |u Laboratório de Bioprocessos, Centro de Biotecnologia, Instituto Butantan, Av. Vital Brasil 1500, 05503-900, São Paulo, SP, Brazil |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/24(2015-12-01), 10447-10456 |x 0175-7598 |q 99:24<10447 |1 2015 |2 99 |o 253 | ||