caa a22 4500 60550234X CHVBK 20210128100605.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00253-015-6928-z doi (NATIONALLICENCE)springer-10.1007/s00253-015-6928-z Capsular polysaccharide production by Streptococcus pneumoniae serotype 1: from strain selection to fed-batch cultivation [Elektronische Daten] [Bruno Marthos, Anne Ferri, Douglas de Figueiredo, Teresa Zangirolami, Viviane Gonçalves] Streptococcus pneumoniae is a human pathogen largely transmitted by aerosols. Vaccines are the main strategy against this pathogen, and the capsular polysaccharide (PS) is its major antigen. S. pneumoniae serotype 1 is associated with large outbreaks and epidemics of invasive diseases. The aims of this work were to screen serotype 1 strains to identify the best PS1 producer, evaluate three peptones for PS1 production, investigate the effects of culture medium components using a design of experiments (DoE), a statistic tool for optimization, and propose a new medium/cultivation strategy. After flask cultivation of nine strains, two that produced high PS1 and biomass values were chosen for further evaluation in the bioreactor, and ST595/01 was chosen as the best PS1 producer strain. Among the peptones tested (Casamino acids, Soytone, and Phytone), the highest PS1 production (298mg/L) was reached with Phytone. Next, DoE (24-1) was performed to evaluate the effects of yeast extract (YE), Phytone, L-asparagine (Asn), and L-glutamine (Gln), yielding the following results: Phytone presented positive effects (p < 0.05) for maximum production of biomass, PS1, acetate, and lactate; YE showed positive effects for biomass and acid production (p < 0.05); Gln exerted a minor positive effect on PS1 yield factor on glucose (p < 0.1); and Asn presented only an effect on acetate production (p < 0.1). Hence, a new culture medium was formulated based on Phytone, YE, and glucose, and batch and fed-batch cultivations were evaluated. The fed-batch cultivation showed almost 2 times the biomass and 2.5 times the PS1 production as the batch culture, and 8-10 times higher PS1 production than has been previously reported. Springer-Verlag Berlin Heidelberg, 2015 Pneumococcus nationallicence Capsular polysaccharide production nationallicence Strain screening nationallicence Peptone selection nationallicence Design of experiments nationallicence Fed-batch cultivation nationallicence Marthos Bruno Laboratório de Bioprocessos, Centro de Biotecnologia, Instituto Butantan, Av. Vital Brasil 1500, 05503-900, São Paulo, SP, Brazil aut Ferri Anne Laboratório de Bioprocessos, Centro de Biotecnologia, Instituto Butantan, Av. Vital Brasil 1500, 05503-900, São Paulo, SP, Brazil aut de Figueiredo Douglas Laboratório de Bioprocessos, Centro de Biotecnologia, Instituto Butantan, Av. Vital Brasil 1500, 05503-900, São Paulo, SP, Brazil aut Zangirolami Teresa Departamento de Engenharia Química, Universidade Federal de São Carlos, Rodovia Washington Luís km 235, 13565-905, São Carlos, SP, Brazil aut Gonçalves Viviane Laboratório de Bioprocessos, Centro de Biotecnologia, Instituto Butantan, Av. Vital Brasil 1500, 05503-900, São Paulo, SP, Brazil aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10447-10456 0175-7598 99:24<10447 2015 99 253 https://doi.org/10.1007/s00253-015-6928-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6928-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Marthos Bruno Laboratório de Bioprocessos, Centro de Biotecnologia, Instituto Butantan, Av. Vital Brasil 1500, 05503-900, São Paulo, SP, Brazil aut NATIONALLICENCE 700 1- Ferri Anne Laboratório de Bioprocessos, Centro de Biotecnologia, Instituto Butantan, Av. Vital Brasil 1500, 05503-900, São Paulo, SP, Brazil aut NATIONALLICENCE 700 1- de Figueiredo Douglas Laboratório de Bioprocessos, Centro de Biotecnologia, Instituto Butantan, Av. Vital Brasil 1500, 05503-900, São Paulo, SP, Brazil aut NATIONALLICENCE 700 1- Zangirolami Teresa Departamento de Engenharia Química, Universidade Federal de São Carlos, Rodovia Washington Luís km 235, 13565-905, São Carlos, SP, Brazil aut NATIONALLICENCE 700 1- Gonçalves Viviane Laboratório de Bioprocessos, Centro de Biotecnologia, Instituto Butantan, Av. Vital Brasil 1500, 05503-900, São Paulo, SP, Brazil aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10447-10456 0175-7598 99:24<10447 2015 99 253