caa a22 4500 605502358 CHVBK 20210128100605.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00253-015-6870-0 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6870-0 Characteristics of a novel thermophilic heterotrophic bacterium, Anoxybacillus contaminans HA, for nitrification-aerobic denitrification [Elektronische Daten] [Jun Chen, Ji Zheng, Yan Li, Hong-hong Hao, Jian-meng Chen] A strain bacterium that is thermophilic, heterotrophic nitrifying, and aerobic denitrifying was isolated and identified as Anoxybacillus contaminans HA for the first time. The identification was based on morphological and physiological characterizations, together with phylogenetic analysis of 16S rDNA sequence. The strain possessed excellent tolerance to high temperatures, with 55°C as its optimum and 60°C as viable. Moreover, NH4 +-N and NO3 −-N could be efficiently removed under thermophilic and solely aerobic conditions, with little intermediate accumulation. Average removal efficiencies of NH4 +-N and NO3 −-N at 55°C reached 71.0 and 74.7%, respectively, with removal rates of 5.83 and 32.08mgl−1h−1, respectively. Single-factor experiments suggested that the optimal conditions for both heterotrophic nitrification and aerobic denitrification were glucose as carbon source, NH4 +-N range of 50-200mgl−1, and wide NO3 −-N range of 200-1000mgl−1. These results indicated that strain HA had heterotrophic nitrification and aerobic denitrification abilities, as well as the notable ability to remove ammonium under thermophilic condition. Thus, this strain has potential application in waste-gas treatment. Springer-Verlag Berlin Heidelberg, 2015 Thermophilic bacterium nationallicence Anoxybacillus contaminans nationallicence Heterotrophic nitrification nationallicence Aerobic denitrification nationallicence NOx removal nationallicence Chen Jun Engineering Research Center of the Ministry of Education for Bioconversion and Biopurification, Zhejiang University of Technology, 310032, Hangzhou, People's Republic of China aut Zheng Ji College of Biological and Environmental Engineering, Zhejiang University of Technology, 310032, Hangzhou, People's Republic of China aut Li Yan College of Biological and Environmental Engineering, Zhejiang University of Technology, 310032, Hangzhou, People's Republic of China aut Hao Hong-hong College of Biological and Environmental Engineering, Zhejiang University of Technology, 310032, Hangzhou, People's Republic of China aut Chen Jian-meng Engineering Research Center of the Ministry of Education for Bioconversion and Biopurification, Zhejiang University of Technology, 310032, Hangzhou, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10695-10702 0175-7598 99:24<10695 2015 99 253 https://doi.org/10.1007/s00253-015-6870-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6870-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Jun Engineering Research Center of the Ministry of Education for Bioconversion and Biopurification, Zhejiang University of Technology, 310032, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zheng Ji College of Biological and Environmental Engineering, Zhejiang University of Technology, 310032, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Li Yan College of Biological and Environmental Engineering, Zhejiang University of Technology, 310032, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Hao Hong-hong College of Biological and Environmental Engineering, Zhejiang University of Technology, 310032, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Chen Jian-meng Engineering Research Center of the Ministry of Education for Bioconversion and Biopurification, Zhejiang University of Technology, 310032, Hangzhou, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10695-10702 0175-7598 99:24<10695 2015 99 253