caa a22 4500 605502366 CHVBK 20210128100605.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00253-015-6942-1 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6942-1 Effects of phosphate addition on methane fermentation in the batch and upflow anaerobic sludge blanket (UASB) reactors [Elektronische Daten] [Sho Suzuki, Masaki Shintani, Zoe Sanchez, Kohei Kimura, Mitsuru Numata, Atsushi Yamazoe, Kazuhide Kimbara] Ammonia inhibition of methane fermentation is one of the leading causes of failure of anaerobic digestion reactors. In a batch anaerobic digestion reactor with 429mM NH3-N/L of ammonia, the addition of 25mM phosphate resulted in an increase in methane production rate. Similar results were obtained with the addition of disodium phosphate in continuous anaerobic digestion using an upflow anaerobic sludge blanket (UASB) reactor. While methane content and production rate decreased in the presence of more than 143mM NH3-N/L of ammonium chloride in UASB, the addition of 5mM disodium phosphate suppressed ammonia inhibition at 214mM NH3-N/L of ammonium chloride. The addition prevented acetate/propionate accumulation, which might be one of the effects of the phosphate on the ammonia inhibition. The effects on the microbial community in the UASB reactor was also assessed, which was composed of Bacteria involved in hydrolysis, acidogenesis, acetogenesis, and dehydrogenation, as well as Archaea carrying out methanogenesis. The change in the microbial community was observed by ammonia inhibition and the addition of phosphate. The change indicates that the suppression of ammonia inhibition by disodium phosphate addition could stimulate the activity of methanogens, reduce shift in bacterial community, and enhance hydrogen-producing bacteria. The addition of phosphate will be an important treatment for future studies of methane fermentation. Springer-Verlag Berlin Heidelberg, 2015 Ammonia inhibition nationallicence Anaerobic digestion nationallicence Methanogen nationallicence Microbial community nationallicence Phosphate nationallicence Methane fermentation nationallicence UASB nationallicence Suzuki Sho Applied Chemistry and Biochemical Engineering Course, Department of Engineering, Graduate School of Integrated Science and Technology, Shizuoka University, 3-5-1 Johoku, Naka-ku, 432-8561, Hamamatsu, Shizuoka, Japan aut Shintani Masaki Applied Chemistry and Biochemical Engineering Course, Department of Engineering, Graduate School of Integrated Science and Technology, Shizuoka University, 3-5-1 Johoku, Naka-ku, 432-8561, Hamamatsu, Shizuoka, Japan aut Sanchez Zoe Applied Chemistry and Biochemical Engineering Course, Department of Engineering, Graduate School of Integrated Science and Technology, Shizuoka University, 3-5-1 Johoku, Naka-ku, 432-8561, Hamamatsu, Shizuoka, Japan aut Kimura Kohei Applied Chemistry and Biochemical Engineering Course, Department of Engineering, Graduate School of Integrated Science and Technology, Shizuoka University, 3-5-1 Johoku, Naka-ku, 432-8561, Hamamatsu, Shizuoka, Japan aut Numata Mitsuru NITE Biological Resource Center, National Institute of Technology and Evaluation, 2-49-10, Nishihara, Shibuya-ku, 151-0066, Tokyo, Japan aut Yamazoe Atsushi NITE Biological Resource Center, National Institute of Technology and Evaluation, 2-49-10, Nishihara, Shibuya-ku, 151-0066, Tokyo, Japan aut Kimbara Kazuhide Applied Chemistry and Biochemical Engineering Course, Department of Engineering, Graduate School of Integrated Science and Technology, Shizuoka University, 3-5-1 Johoku, Naka-ku, 432-8561, Hamamatsu, Shizuoka, Japan aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10457-10466 0175-7598 99:24<10457 2015 99 253 https://doi.org/10.1007/s00253-015-6942-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6942-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Suzuki Sho Applied Chemistry and Biochemical Engineering Course, Department of Engineering, Graduate School of Integrated Science and Technology, Shizuoka University, 3-5-1 Johoku, Naka-ku, 432-8561, Hamamatsu, Shizuoka, Japan aut NATIONALLICENCE 700 1- Shintani Masaki Applied Chemistry and Biochemical Engineering Course, Department of Engineering, Graduate School of Integrated Science and Technology, Shizuoka University, 3-5-1 Johoku, Naka-ku, 432-8561, Hamamatsu, Shizuoka, Japan aut NATIONALLICENCE 700 1- Sanchez Zoe Applied Chemistry and Biochemical Engineering Course, Department of Engineering, Graduate School of Integrated Science and Technology, Shizuoka University, 3-5-1 Johoku, Naka-ku, 432-8561, Hamamatsu, Shizuoka, Japan aut NATIONALLICENCE 700 1- Kimura Kohei Applied Chemistry and Biochemical Engineering Course, Department of Engineering, Graduate School of Integrated Science and Technology, Shizuoka University, 3-5-1 Johoku, Naka-ku, 432-8561, Hamamatsu, Shizuoka, Japan aut NATIONALLICENCE 700 1- Numata Mitsuru NITE Biological Resource Center, National Institute of Technology and Evaluation, 2-49-10, Nishihara, Shibuya-ku, 151-0066, Tokyo, Japan aut NATIONALLICENCE 700 1- Yamazoe Atsushi NITE Biological Resource Center, National Institute of Technology and Evaluation, 2-49-10, Nishihara, Shibuya-ku, 151-0066, Tokyo, Japan aut NATIONALLICENCE 700 1- Kimbara Kazuhide Applied Chemistry and Biochemical Engineering Course, Department of Engineering, Graduate School of Integrated Science and Technology, Shizuoka University, 3-5-1 Johoku, Naka-ku, 432-8561, Hamamatsu, Shizuoka, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10457-10466 0175-7598 99:24<10457 2015 99 253