Pyrosequencing analysis of bacterial communities in biofilms from different pipe materials in a city drinking water distribution system of East China

Verfasser / Beitragende:
[Hongxing Ren, Wei Wang, Yan Liu, Shuai Liu, Liping Lou, Dongqing Cheng, Xiaofang He, Xiaoyan Zhou, Shangde Qiu, Liusong Fu, Jingqing Liu, Baolan Hu]
Ort, Verlag, Jahr:
2015
Enthalten in:
Applied Microbiology and Biotechnology, 99/24(2015-12-01), 10713-10724
Format:
Artikel (online)
ID: 605502382
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024 7 0 |a 10.1007/s00253-015-6885-6  |2 doi 
035 |a (NATIONALLICENCE)springer-10.1007/s00253-015-6885-6 
245 0 0 |a Pyrosequencing analysis of bacterial communities in biofilms from different pipe materials in a city drinking water distribution system of East China  |h [Elektronische Daten]  |c [Hongxing Ren, Wei Wang, Yan Liu, Shuai Liu, Liping Lou, Dongqing Cheng, Xiaofang He, Xiaoyan Zhou, Shangde Qiu, Liusong Fu, Jingqing Liu, Baolan Hu] 
520 3 |a Biofilms in drinking water distribution systems (DWDSs) could cause several types of problems, such as the deterioration of water quality, corrosion of pipe walls, and potential proliferation of opportunistic pathogens. In this study, ten biofilm samples from different pipe materials, including ductile cast iron pipe (DCIP), gray cast iron pipe (GCIP), galvanized steel pipe (GSP), stainless steel clad pipe (SSCP), and polyvinyl chloride (PVC), were collected from an actual DWDS to investigate the effect of pipe material on bacterial community. Real-time quantitative polymerase chain reaction (qPCR) and culture-based method were used to quantify bacteria. 454 pyrosequencing was used for bacterial community analysis. The results showed that the numbers of total bacteria and culturable heterotrophic bacteria from iron pipes were higher than that in PVC, while the numbers of Shigella and vibrios were low in biofilms from iron pipes. Bacterial community analysis showed that Hyphomicrobium or Desulfovibrio were the predominant microorganism in iron pipes, whereas Sphingomonas or Pseudomonas were dominant in other types of pipe. This study revealed differences in bacterial communities in biofilms among different pipe materials, and the results were useful for pipeline material selection in DWDSs. 
540 |a Springer-Verlag Berlin Heidelberg, 2015 
690 7 |a Drinking water distribution system  |2 nationallicence 
690 7 |a Biofilms  |2 nationallicence 
690 7 |a Pipe materials  |2 nationallicence 
690 7 |a Bacterial community  |2 nationallicence 
700 1 |a Ren  |D Hongxing  |u Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China  |4 aut 
700 1 |a Wang  |D Wei  |u Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China  |4 aut 
700 1 |a Liu  |D Yan  |u Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China  |4 aut 
700 1 |a Liu  |D Shuai  |u Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China  |4 aut 
700 1 |a Lou  |D Liping  |u Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China  |4 aut 
700 1 |a Cheng  |D Dongqing  |u Zhejiang Chinese Medical University, 310053, Hangzhou, Zhejiang Province, China  |4 aut 
700 1 |a He  |D Xiaofang  |u Shaoxing Water Environmental Science Institute Co. Ltd, 312000, Shaoxing, China  |4 aut 
700 1 |a Zhou  |D Xiaoyan  |u Shaoxing Water Environmental Science Institute Co. Ltd, 312000, Shaoxing, China  |4 aut 
700 1 |a Qiu  |D Shangde  |u Shaoxing Water Environmental Science Institute Co. Ltd, 312000, Shaoxing, China  |4 aut 
700 1 |a Fu  |D Liusong  |u Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China  |4 aut 
700 1 |a Liu  |D Jingqing  |u College of Civil Engineering and Architecture, Zhejiang University, 310058, Hangzhou, China  |4 aut 
700 1 |a Hu  |D Baolan  |u Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China  |4 aut 
773 0 |t Applied Microbiology and Biotechnology  |d Springer Berlin Heidelberg  |g 99/24(2015-12-01), 10713-10724  |x 0175-7598  |q 99:24<10713  |1 2015  |2 99  |o 253 
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950 |B NATIONALLICENCE  |P 700  |E 1-  |a Ren  |D Hongxing  |u Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Wang  |D Wei  |u Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Liu  |D Yan  |u Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Liu  |D Shuai  |u Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Lou  |D Liping  |u Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Cheng  |D Dongqing  |u Zhejiang Chinese Medical University, 310053, Hangzhou, Zhejiang Province, China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a He  |D Xiaofang  |u Shaoxing Water Environmental Science Institute Co. Ltd, 312000, Shaoxing, China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Zhou  |D Xiaoyan  |u Shaoxing Water Environmental Science Institute Co. Ltd, 312000, Shaoxing, China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Qiu  |D Shangde  |u Shaoxing Water Environmental Science Institute Co. Ltd, 312000, Shaoxing, China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Fu  |D Liusong  |u Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Liu  |D Jingqing  |u College of Civil Engineering and Architecture, Zhejiang University, 310058, Hangzhou, China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Hu  |D Baolan  |u Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China  |4 aut 
950 |B NATIONALLICENCE  |P 773  |E 0-  |t Applied Microbiology and Biotechnology  |d Springer Berlin Heidelberg  |g 99/24(2015-12-01), 10713-10724  |x 0175-7598  |q 99:24<10713  |1 2015  |2 99  |o 253