caa a22 4500 605502382 CHVBK 20210128100605.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00253-015-6885-6 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6885-6 Pyrosequencing analysis of bacterial communities in biofilms from different pipe materials in a city drinking water distribution system of East China [Elektronische Daten] [Hongxing Ren, Wei Wang, Yan Liu, Shuai Liu, Liping Lou, Dongqing Cheng, Xiaofang He, Xiaoyan Zhou, Shangde Qiu, Liusong Fu, Jingqing Liu, Baolan Hu] Biofilms in drinking water distribution systems (DWDSs) could cause several types of problems, such as the deterioration of water quality, corrosion of pipe walls, and potential proliferation of opportunistic pathogens. In this study, ten biofilm samples from different pipe materials, including ductile cast iron pipe (DCIP), gray cast iron pipe (GCIP), galvanized steel pipe (GSP), stainless steel clad pipe (SSCP), and polyvinyl chloride (PVC), were collected from an actual DWDS to investigate the effect of pipe material on bacterial community. Real-time quantitative polymerase chain reaction (qPCR) and culture-based method were used to quantify bacteria. 454 pyrosequencing was used for bacterial community analysis. The results showed that the numbers of total bacteria and culturable heterotrophic bacteria from iron pipes were higher than that in PVC, while the numbers of Shigella and vibrios were low in biofilms from iron pipes. Bacterial community analysis showed that Hyphomicrobium or Desulfovibrio were the predominant microorganism in iron pipes, whereas Sphingomonas or Pseudomonas were dominant in other types of pipe. This study revealed differences in bacterial communities in biofilms among different pipe materials, and the results were useful for pipeline material selection in DWDSs. Springer-Verlag Berlin Heidelberg, 2015 Drinking water distribution system nationallicence Biofilms nationallicence Pipe materials nationallicence Bacterial community nationallicence Ren Hongxing Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut Wang Wei Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut Liu Yan Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut Liu Shuai Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut Lou Liping Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut Cheng Dongqing Zhejiang Chinese Medical University, 310053, Hangzhou, Zhejiang Province, China aut He Xiaofang Shaoxing Water Environmental Science Institute Co. Ltd, 312000, Shaoxing, China aut Zhou Xiaoyan Shaoxing Water Environmental Science Institute Co. Ltd, 312000, Shaoxing, China aut Qiu Shangde Shaoxing Water Environmental Science Institute Co. Ltd, 312000, Shaoxing, China aut Fu Liusong Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut Liu Jingqing College of Civil Engineering and Architecture, Zhejiang University, 310058, Hangzhou, China aut Hu Baolan Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10713-10724 0175-7598 99:24<10713 2015 99 253 https://doi.org/10.1007/s00253-015-6885-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6885-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ren Hongxing Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Wang Wei Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Liu Yan Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Liu Shuai Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Lou Liping Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Cheng Dongqing Zhejiang Chinese Medical University, 310053, Hangzhou, Zhejiang Province, China aut NATIONALLICENCE 700 1- He Xiaofang Shaoxing Water Environmental Science Institute Co. Ltd, 312000, Shaoxing, China aut NATIONALLICENCE 700 1- Zhou Xiaoyan Shaoxing Water Environmental Science Institute Co. Ltd, 312000, Shaoxing, China aut NATIONALLICENCE 700 1- Qiu Shangde Shaoxing Water Environmental Science Institute Co. Ltd, 312000, Shaoxing, China aut NATIONALLICENCE 700 1- Fu Liusong Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Liu Jingqing College of Civil Engineering and Architecture, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Hu Baolan Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10713-10724 0175-7598 99:24<10713 2015 99 253