caa a22 4500 605502390 CHVBK 20210128100605.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00253-015-6925-2 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6925-2 The effects of upaB deletion and the double/triple deletion of upaB , aatA , and aatB genes on pathogenicity of avian pathogenic Escherichia coli [Elektronische Daten] [Xiang-kai Zhu-Ge, Zi-hao Pan, Fang Tang, Xiang Mao, Lin Hu, Shao-hui Wang, Bin Xu, Cheng-ping Lu, Hong-jie Fan, Jian-jun Dai] Autotransporters (ATs) are associated with pathogenesis of Avian Pathogenic Escherichia coli (APEC). The molecular characterization of APEC ATs can provide insights about their relevance to APEC pathogenesis. Here, we characterized a conventional autotransporter UpaB in APEC DE205B genome. The upaB existed in 41.9% of 236 APEC isolates and was predominantly associated with ECOR B2 and D. Our studies showed that UpaB mediates the DE205B adhesion in DF-1 cells, and enhances autoaggregation and biofilm formation of fimbria-negative E. coli AAEC189 (MG1655Δfim) in vitro. Deletion of upaB of DE205B attenuates the virulence in duck model and early colonization in the duck lungs during APEC systemic infection. Furthermore, double and triple deletion of upaB, aatA, and aatB genes cumulatively attenuated DE205B adhesion in DF-1 cells, accompanying with decreased 50% lethal dose (LD50) in duck model and the early colonization in the duck lungs. However, DE205BΔupaB/ΔaatA/ΔaatB might "compensate” the influence of gene deletion by upregulating the expression of fimbrial adhesin genes yqiL, yadN, and vacuolating autotransporter vat during early colonization of APEC. Finally, we demonstrated that vaccination with recombinant UpaB, AatA, and AatB proteins conferred protection against colisepticemia caused by DE205B infection in duck model. Springer-Verlag Berlin Heidelberg, 2015 APEC nationallicence Autotransporter UPAB nationallicence Pathogenicity nationallicence Double/triple deletion nationallicence Vaccination nationallicence Zhu-Ge Xiang-kai Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural, University, No.1 Weigang road, 210095, Nanjing, Jiangsu Province, China aut Pan Zi-hao Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural, University, No.1 Weigang road, 210095, Nanjing, Jiangsu Province, China aut Tang Fang Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural, University, No.1 Weigang road, 210095, Nanjing, Jiangsu Province, China aut Mao Xiang Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 200241, Shanghai, China aut Hu Lin Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural, University, No.1 Weigang road, 210095, Nanjing, Jiangsu Province, China aut Wang Shao-hui Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 200241, Shanghai, China aut Xu Bin Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural, University, No.1 Weigang road, 210095, Nanjing, Jiangsu Province, China aut Lu Cheng-ping Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural, University, No.1 Weigang road, 210095, Nanjing, Jiangsu Province, China aut Fan Hong-jie Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural, University, No.1 Weigang road, 210095, Nanjing, Jiangsu Province, China aut Dai Jian-jun Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural, University, No.1 Weigang road, 210095, Nanjing, Jiangsu Province, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10639-10654 0175-7598 99:24<10639 2015 99 253 https://doi.org/10.1007/s00253-015-6925-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6925-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhu-Ge Xiang-kai Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural, University, No.1 Weigang road, 210095, Nanjing, Jiangsu Province, China aut NATIONALLICENCE 700 1- Pan Zi-hao Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural, University, No.1 Weigang road, 210095, Nanjing, Jiangsu Province, China aut NATIONALLICENCE 700 1- Tang Fang Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural, University, No.1 Weigang road, 210095, Nanjing, Jiangsu Province, China aut NATIONALLICENCE 700 1- Mao Xiang Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 200241, Shanghai, China aut NATIONALLICENCE 700 1- Hu Lin Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural, University, No.1 Weigang road, 210095, Nanjing, Jiangsu Province, China aut NATIONALLICENCE 700 1- Wang Shao-hui Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 200241, Shanghai, China aut NATIONALLICENCE 700 1- Xu Bin Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural, University, No.1 Weigang road, 210095, Nanjing, Jiangsu Province, China aut NATIONALLICENCE 700 1- Lu Cheng-ping Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural, University, No.1 Weigang road, 210095, Nanjing, Jiangsu Province, China aut NATIONALLICENCE 700 1- Fan Hong-jie Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural, University, No.1 Weigang road, 210095, Nanjing, Jiangsu Province, China aut NATIONALLICENCE 700 1- Dai Jian-jun Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural, University, No.1 Weigang road, 210095, Nanjing, Jiangsu Province, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10639-10654 0175-7598 99:24<10639 2015 99 253