caa a22 4500 605502439 CHVBK 20210128100605.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00253-015-6916-3 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6916-3 Effect of inoculum and sulfide type on simultaneous hydrogen sulfide removal from biogas and nitrogen removal from swine slurry and microbial mechanism [Elektronische Daten] [Lan Wang, Benping Wei, Ziai Chen, Liangwei Deng, Li Song, Shuang Wang, Dan Zheng, Yi Liu, Xiaodong Pu, Yunhong Zhang] Four reactors were initiated to study the effect of inoculum and sulfide type on the simultaneous hydrogen sulfide removal from biogas and nitrogen removal from swine slurry (Ssu-Nir) process. Anaerobic sludge, aerobic sludge, and water were used as inocula, and Na2S and biogas were used as a sulfide substrate, respectively. Additionally, 454 pyrosequencing of the 16S rRNA gene was used to explore the bacterial diversity. The results showed that sulfur-oxidizing bacteria (Thiobacillus, 42.2-84.4%) were dominant in Ssu-Nir process and led to the excellent performance. Aerobic sludge was more suitable for inoculation of the Ssu-Nir process because it is better for rapidly enriching dominant sulfur-oxidizing bacteria (Thiobacillus, 54.4%), denitrifying sulfur-oxidizing bacteria (40.0%) and denitrifiers (23.9%). Lower S2− removal efficiency (72.6%) and NO3 − removal efficiency (<90%) of the Ssu-Nir process were obtained using biogas as a sulfide substrate than when Na2S was used. For the Ssu-Nir process with biogas as the sulfide substrate, limiting H2S absorption caused a high relative abundance of sulfur-oxidizing bacteria, Thiobacillus (84.8%) and Thiobacillus sayanicus (39.6%), which in turn led to low relative abundance of denitrifiers (1.6%) and denitrifying sulfur-oxidizing bacteria (24.4%), low NO3 − removal efficiency, and eventually poor performance. Springer-Verlag Berlin Heidelberg, 2015 Nitrogen removal nationallicence Hydrogen sulfide removal nationallicence Biogas nationallicence Swine slurry nationallicence 454 pyrosequencing nationallicence Bacterial diversity nationallicence Wang Lan Biogas Institute of Ministry of Agriculture, 610041, Chengdu, China aut Wei Benping Biogas Institute of Ministry of Agriculture, 610041, Chengdu, China aut Chen Ziai Biogas Institute of Ministry of Agriculture, 610041, Chengdu, China aut Deng Liangwei Biogas Institute of Ministry of Agriculture, 610041, Chengdu, China aut Song Li Biogas Institute of Ministry of Agriculture, 610041, Chengdu, China aut Wang Shuang Biogas Institute of Ministry of Agriculture, 610041, Chengdu, China aut Zheng Dan Biogas Institute of Ministry of Agriculture, 610041, Chengdu, China aut Liu Yi Biogas Institute of Ministry of Agriculture, 610041, Chengdu, China aut Pu Xiaodong Biogas Institute of Ministry of Agriculture, 610041, Chengdu, China aut Zhang Yunhong Biogas Institute of Ministry of Agriculture, 610041, Chengdu, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10793-10803 0175-7598 99:24<10793 2015 99 253 https://doi.org/10.1007/s00253-015-6916-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6916-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Lan Biogas Institute of Ministry of Agriculture, 610041, Chengdu, China aut NATIONALLICENCE 700 1- Wei Benping Biogas Institute of Ministry of Agriculture, 610041, Chengdu, China aut NATIONALLICENCE 700 1- Chen Ziai Biogas Institute of Ministry of Agriculture, 610041, Chengdu, China aut NATIONALLICENCE 700 1- Deng Liangwei Biogas Institute of Ministry of Agriculture, 610041, Chengdu, China aut NATIONALLICENCE 700 1- Song Li Biogas Institute of Ministry of Agriculture, 610041, Chengdu, China aut NATIONALLICENCE 700 1- Wang Shuang Biogas Institute of Ministry of Agriculture, 610041, Chengdu, China aut NATIONALLICENCE 700 1- Zheng Dan Biogas Institute of Ministry of Agriculture, 610041, Chengdu, China aut NATIONALLICENCE 700 1- Liu Yi Biogas Institute of Ministry of Agriculture, 610041, Chengdu, China aut NATIONALLICENCE 700 1- Pu Xiaodong Biogas Institute of Ministry of Agriculture, 610041, Chengdu, China aut NATIONALLICENCE 700 1- Zhang Yunhong Biogas Institute of Ministry of Agriculture, 610041, Chengdu, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10793-10803 0175-7598 99:24<10793 2015 99 253