caa a22 4500 605502447 CHVBK 20210128100605.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00253-015-6901-x doi (NATIONALLICENCE)springer-10.1007/s00253-015-6901-x Cadmium resistance mechanism in Escherichia coli P4 and its potential use to bioremediate environmental cadmium [Elektronische Daten] [Zaman Khan, Muhammad Nisar, Syed Hussain, Muhammad Arshad, Abdul Rehman] A cadmium-resistant bacterium was isolated from industrial wastewater and identified as Escherichia coli (dubbed as P4) on the basis of morphological, biochemical tests and 16S rRNA ribotyping. It showed optimum growth at 30°C and pH 7. E. coli P4 found to resist Cd+2 (10.6mM) as well as Zn+2 (4.4mM), Pb+2 (17mM), Cu+2 (3.5mM), Cr+6 (4.4mM), As+2 (10.6mM), and Hg+2 (0.53mM). It could remove 18.8, 37, and 56% Cd+2 from aqueous medium after 48, 96, and 144h, respectively. Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), and Energy-dispersive X-ray (EDX) analysis also confirmed the biosorption of Cd+2 by E. coli P4. However, temperature and pH were found to be the most critical factors in biosorption of Cd+2 by E. coli P4. Cd+2 stress altered E. coli P4 cell physiology analyzed by measuring glutathione (GSH) and non-protein thiol (cysteine) levels which were increased up to 130 and 48%, respectively. Quantitative real-time polymerase chain reaction (qRT-PCR) showed alteration in the expression levels of ftsZ, mutS, clpB, ef-tu, and dnaK genes in the presence of Cd+2. Total protein profiles of E. coli P4 in the absence and presence of Cd+2 were compared by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), which showed remarkable difference in the banding pattern. czcB gene, a component of czcCBA operon, was amplified from genomic DNA which suggested the chromosomal-borne Cd+2 resistance in E. coli P4. Furthermore, it harbors smtAB gene which plays a significant role in Cd+2 resistance. Springer-Verlag Berlin Heidelberg, 2015 Cadmium-resistant E. coli nationallicence Biosorption nationallicence Glutathione nationallicence Metallothionein nationallicence czcB gene nationallicence Khan Zaman Department of Microbiology and Molecular Genetics, University of the Punjab, New Campus, Quaid Azam Campus, 54590, Lahore, Pakistan aut Nisar Muhammad Department of Microbiology, Govt. College University Faislabad, Faislabad, Pakistan aut Hussain Syed Department of Chemistry (SZH), SBA School of Sciences and Engineering (SBASSE) Lahore, University of Management Sciences (LUMS), DHA, 54792, Lahore Cantt, Pakistan aut Arshad Muhammad Department of Biology, University of Management Sciences, (LUMS), DHA, Lahore Cantt, 54792, Lahore, Pakistan aut Rehman Abdul Department of Microbiology and Molecular Genetics, University of the Punjab, New Campus, Quaid Azam Campus, 54590, Lahore, Pakistan aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10745-10757 0175-7598 99:24<10745 2015 99 253 https://doi.org/10.1007/s00253-015-6901-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6901-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Khan Zaman Department of Microbiology and Molecular Genetics, University of the Punjab, New Campus, Quaid Azam Campus, 54590, Lahore, Pakistan aut NATIONALLICENCE 700 1- Nisar Muhammad Department of Microbiology, Govt. College University Faislabad, Faislabad, Pakistan aut NATIONALLICENCE 700 1- Hussain Syed Department of Chemistry (SZH), SBA School of Sciences and Engineering (SBASSE) Lahore, University of Management Sciences (LUMS), DHA, 54792, Lahore Cantt, Pakistan aut NATIONALLICENCE 700 1- Arshad Muhammad Department of Biology, University of Management Sciences, (LUMS), DHA, Lahore Cantt, 54792, Lahore, Pakistan aut NATIONALLICENCE 700 1- Rehman Abdul Department of Microbiology and Molecular Genetics, University of the Punjab, New Campus, Quaid Azam Campus, 54590, Lahore, Pakistan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10745-10757 0175-7598 99:24<10745 2015 99 253