caa a22 4500 605502463 CHVBK 20210128100606.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00253-015-6939-9 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6939-9 Nitrogen removal from wastewater by anaerobic methane-driven denitrification in a lab-scale reactor: heterotrophic denitrifiers associated with denitrifying methanotrophs [Elektronische Daten] [Zhanfei He, Jiaqi Wang, Xu Zhang, Chaoyang Cai, Sha Geng, Ping Zheng, Xinhua Xu, Baolan Hu] Nitrite-dependent anaerobic methane oxidation (n-damo) is a newly discovered bioprocess that reduces nitrite to dinitrogen with methane as electron donor, which has promising potential to remove nitrogen from wastewater. In this work, a lab-scale sequencing batch reactor (SBR) was operated for 609days with methane as the sole external electron donor. In the SBR, nitrite in synthetic wastewater was removed continuously; the final volumetric nitrogen removal rate was 12.22±0.02mgNL−1day−1 and the percentage of nitrogen removal was 98.5±0.2%. Microbial community analysis indicated that denitrifying methanotrophs dominated (60-70%) the population of the final sludge. Notably, activity testing and microbial analysis both suggested that heterotrophic denitrifiers existed in the reactor throughout the operation period. After 609days, the activity testing indicated the nitrogen removal percentage of heterotrophic denitrification was 17±2% and that of n-damo was 83±2%. A possible mutualism may be developed between the dominated denitrifying methanotrophs and the associated heterotrophs through cross-feed. Heterotrophs may live on the microbial products excreted by denitrifying methanotrophs and provide growth factors that are required by denitrifying methanotrophs. Springer-Verlag Berlin Heidelberg, 2015 Biological nitrogen removal nationallicence Methane-driven denitrification nationallicence n-damo nationallicence Denitrifying methanotroph nationallicence Heterotrophic denitrification nationallicence He Zhanfei Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut Wang Jiaqi Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut Zhang Xu Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut Cai Chaoyang Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut Geng Sha Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut Zheng Ping Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut Xu Xinhua Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut Hu Baolan Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10853-10860 0175-7598 99:24<10853 2015 99 253 https://doi.org/10.1007/s00253-015-6939-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6939-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- He Zhanfei Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Wang Jiaqi Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Zhang Xu Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Cai Chaoyang Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Geng Sha Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Zheng Ping Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Xu Xinhua Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Hu Baolan Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10853-10860 0175-7598 99:24<10853 2015 99 253