APC targeting enhances immunogenicity of a novel multistage Fc-fusion tuberculosis vaccine in mice
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| 008 | 210128e20151201xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-015-6952-z |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6952-z | ||
| 245 | 0 | 0 | |a APC targeting enhances immunogenicity of a novel multistage Fc-fusion tuberculosis vaccine in mice |h [Elektronische Daten] |c [Saman Soleimanpour, Hadi Farsiani, Arman Mosavat, Kiarash Ghazvini, Mohammad Eydgahi, Mojtaba Sankian, Hamid Sadeghian, Zahra Meshkat, Seyed Rezaee] |
| 520 | 3 | |a Numerous studies have demonstrated that targeting immunogens to FcγR on antigen-presenting cells (APCs) can selectively uptake and increase cellular immunity in vitro and in vivo. Therefore, the present study was conducted to evaluate immunogenicity of a novel multistage tuberculosis vaccine, a combination of an early and a dormant immunogenic protein, ESAT6 and HspX, fused to Fcγ2a fragment of mouse IgG2a to target all forms of tuberculosis. Codon-optimized genes consisting of ESAT6, a linker, and HspX fused either to mouse Fcγ2a (ESAT6:HspX:mFcγ2a) or 6× His-tag (ESAT6:HspX:His) were synthesized. The resulting proteins were then produced in Pichia pastoris. The fusion proteins were separately emulsified in dimethyldioctadecylammonium bromide(DDA)-trehalose-6,6-dibehenate(TDB) adjuvant, and their immunogenicity with and without bacille Calmette-Guérin (BCG) was assessed in C57BL/6 mice. Th1, Th2, Th17, and T-reg cytokine patterns were evaluated using the ELISA method. Both multistage vaccines induced very strong IL-12 and IFN-γ secretion from splenic cells; the Fc-tagged subunit vaccine induced a more effective Th1 immune response (IFN-γ, 910pg/mL, and IL-12, 854pg/mL) with a very low increase in IL-17 (∼0.1pg/mL) and IL-4 (37pg/mL) and a mild increase in TGF-β (543pg/mL) compared to the BCG or ESAT6:HspX:His primed and boosted groups. The production of IFN-γ to ESAT6:HspX:Fcγ2a was very consistent and showed an increasing trend for IL-12 compared to the BCG or ESAT6:HspX:His primed and boosted groups. Fcγ2a used as a delivery vehicle supported the idea of selective uptake, inducing cross-presentation and forming a proper anti-tuberculosis response in context of Th1/Th2 and Th17/T-reg balances, which is important for protection and prevention of damage. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Tuberculosis |2 nationallicence | |
| 690 | 7 | |a APC-targeting |2 nationallicence | |
| 690 | 7 | |a Fc-fusion vaccine |2 nationallicence | |
| 690 | 7 | |a ESAT-6 |2 nationallicence | |
| 690 | 7 | |a HspX |2 nationallicence | |
| 700 | 1 | |a Soleimanpour |D Saman |u Microbiology & Virology Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran |4 aut | |
| 700 | 1 | |a Farsiani |D Hadi |u Biotechnology Research Center, Semnan University of Medical Sciences, Semnan, Iran |4 aut | |
| 700 | 1 | |a Mosavat |D Arman |u Microbiology & Virology Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran |4 aut | |
| 700 | 1 | |a Ghazvini |D Kiarash |u Microbiology & Virology Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran |4 aut | |
| 700 | 1 | |a Eydgahi |D Mohammad |u Biotechnology Research Center, Semnan University of Medical Sciences, Semnan, Iran |4 aut | |
| 700 | 1 | |a Sankian |D Mojtaba |u Immunobiochemistry Lab, Immunology Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran |4 aut | |
| 700 | 1 | |a Sadeghian |D Hamid |u Organic Chemistry, Department of Laboratory Sciences, Mashhad University of Medical Sciences, Mashhad, Iran |4 aut | |
| 700 | 1 | |a Meshkat |D Zahra |u Microbiology & Virology Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran |4 aut | |
| 700 | 1 | |a Rezaee |D Seyed |u Microbiology & Virology Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/24(2015-12-01), 10467-10480 |x 0175-7598 |q 99:24<10467 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6952-z |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6952-z |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Soleimanpour |D Saman |u Microbiology & Virology Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Farsiani |D Hadi |u Biotechnology Research Center, Semnan University of Medical Sciences, Semnan, Iran |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Mosavat |D Arman |u Microbiology & Virology Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ghazvini |D Kiarash |u Microbiology & Virology Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Eydgahi |D Mohammad |u Biotechnology Research Center, Semnan University of Medical Sciences, Semnan, Iran |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sankian |D Mojtaba |u Immunobiochemistry Lab, Immunology Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sadeghian |D Hamid |u Organic Chemistry, Department of Laboratory Sciences, Mashhad University of Medical Sciences, Mashhad, Iran |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Meshkat |D Zahra |u Microbiology & Virology Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Rezaee |D Seyed |u Microbiology & Virology Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/24(2015-12-01), 10467-10480 |x 0175-7598 |q 99:24<10467 |1 2015 |2 99 |o 253 | ||