caa a22 4500 605502498 CHVBK 20210128100606.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00253-015-6945-y doi (NATIONALLICENCE)springer-10.1007/s00253-015-6945-y Metagenome approaches revealed a biological prospect for improvement on mesophilic cellulose degradation [Elektronische Daten] [Yubo Wang, Yu Xia, Feng Ju, Tong Zhang] Improvement on the bioconversion of cellulosic biomass depends much on the expanded knowledge on the underlying microbial structure and the relevant genetic information. In this study, metagenomic analysis was applied to characterize an enriched mesophilic cellulose-converting consortium, to explore its cellulose-hydrolyzing genes, and to discern genes involved in methanogenesis. Cellulose conversion efficiency of the mesophilic consortium enriched in this study was around 70%. Apart from methane, acetate was the major fermentation product in the liquid phase, while propionate and butyrate were also detected at relatively high concentrations. With the intention to uncover the biological factors that might shape the varying cellulose conversion efficiency at different temperatures, results of this mesophilic consortium were then compared with that of a previously reported thermophilic cellulose-converting consortium. It was found that the mesophilic consortium harbored a larger pool of putative carbohydrate-active genes, with 813 of them in 54 GH modules and 607 genes in 13 CBM modules. Methanobacteriaceae and Methanosaetaceae were the two methanogen families identified, with a preponderance of the hydrogenotrophic Methanobacteriaceae. In contrast to its relatively high diversity and high abundance of carbohydrate-active genes, the abundance of genes involved in the methane metabolism was comparatively lower in the mesophilic consortium. A biological enhancement on the methanogenic process might serve as an effective option for the improvement of the cellulose bioconversion at mesophilic temperature. Springer-Verlag Berlin Heidelberg, 2015 Mesophilic nationallicence Cellulose degradation nationallicence Metagenomics nationallicence Methanogenesis nationallicence Wang Yubo Environmental Biotechnology Lab, The University of Hong Kong SAR, Pokfulam Road, Hong Kong, China aut Xia Yu Environmental Biotechnology Lab, The University of Hong Kong SAR, Pokfulam Road, Hong Kong, China aut Ju Feng Environmental Biotechnology Lab, The University of Hong Kong SAR, Pokfulam Road, Hong Kong, China aut Zhang Tong Environmental Biotechnology Lab, The University of Hong Kong SAR, Pokfulam Road, Hong Kong, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10871-10879 0175-7598 99:24<10871 2015 99 253 https://doi.org/10.1007/s00253-015-6945-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6945-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Yubo Environmental Biotechnology Lab, The University of Hong Kong SAR, Pokfulam Road, Hong Kong, China aut NATIONALLICENCE 700 1- Xia Yu Environmental Biotechnology Lab, The University of Hong Kong SAR, Pokfulam Road, Hong Kong, China aut NATIONALLICENCE 700 1- Ju Feng Environmental Biotechnology Lab, The University of Hong Kong SAR, Pokfulam Road, Hong Kong, China aut NATIONALLICENCE 700 1- Zhang Tong Environmental Biotechnology Lab, The University of Hong Kong SAR, Pokfulam Road, Hong Kong, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10871-10879 0175-7598 99:24<10871 2015 99 253