caa a22 4500 605502528 CHVBK 20210128100606.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00253-015-6908-3 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6908-3 Re-cultivation of Neochloris oleoabundans in exhausted autotrophic and mixotrophic media: the potential role of polyamines and free fatty acids [Elektronische Daten] [Alessandra Sabia, Costanza Baldisserotto, Stefania Biondi, Roberta Marchesini, Paola Tedeschi, Annalisa Maietti, Martina Giovanardi, Lorenzo Ferroni, Simonetta Pancaldi] Neochloris oleoabundans (Chlorophyta) is widely considered one of the most promising microalgae for biotechnological applications. However, the large-scale production of microalgae requires large amounts of water. In this perspective, the possibility of using exhausted growth media for the re-cultivation of N. oleoabundans was investigated in order to simultaneously make the cultivation more economically feasible and environmentally sustainable. Experiments were performed by testing the following media: autotrophic exhausted medium (E+) and mixotrophic exhausted medium after cultivation with glucose (EG+) of N. oleoabundans cells grown in a 20-L photobioreactor (PBR). Both exhausted media were replenished with the same amounts of nitrate and phosphate as the control brackish medium (C). Growth kinetics, nitrate and phosphate consumption, photosynthetic pigments content, photosynthetic efficiency, cell morphology, and lipid production were evaluated. Moreover, the free fatty acid (FFA) composition of exhausted media and the polyamine (PA) concentrations of both algae and media were analyzed in order to test if some molecules, released into the medium, could influence algal growth and metabolism. Results showed that N. oleoabundans can efficiently grow in both exhausted media, if appropriately replenished with the main nutrients (E+ and EG+), especially in E+ and to the same extent as in C medium. Growth promotion of N. oleoabundans was attributed to PAs and alteration of the photosynthetic apparatus to FFAs. Taken together, results show that recycling growth medium is a suitable solution to obtain good N. oleoabundans biomass concentrations, while providing a more sustainable ecological impact on water resources. Springer-Verlag Berlin Heidelberg, 2015 Neochloris oleoabundans nationallicence Recycling culture media nationallicence Photosynthetic apparatus nationallicence Biomass production nationallicence Polyamines nationallicence Free fatty acids nationallicence Sabia Alessandra Department of Life Sciences and Biotechnology, University of Ferrara, Corso Ercole I d'Este 32, 44121, Ferrara, Italy aut Baldisserotto Costanza Department of Life Sciences and Biotechnology, University of Ferrara, Corso Ercole I d'Este 32, 44121, Ferrara, Italy aut Biondi Stefania Department of Biological, Geological and Environmental Sciences, University of Bologna, via Irnerio 42, 40126, Bologna, Italy aut Marchesini Roberta Department of Life Sciences and Biotechnology, University of Ferrara, Corso Ercole I d'Este 32, 44121, Ferrara, Italy aut Tedeschi Paola Department of Chemical and Pharmaceutical Science, University of Ferrara, via Fossato di Mortara 17, 44121, Ferrara, Italy aut Maietti Annalisa Department of Chemical and Pharmaceutical Science, University of Ferrara, via Fossato di Mortara 17, 44121, Ferrara, Italy aut Giovanardi Martina Department of Life Sciences and Biotechnology, University of Ferrara, Corso Ercole I d'Este 32, 44121, Ferrara, Italy aut Ferroni Lorenzo Department of Life Sciences and Biotechnology, University of Ferrara, Corso Ercole I d'Este 32, 44121, Ferrara, Italy aut Pancaldi Simonetta Department of Life Sciences and Biotechnology, University of Ferrara, Corso Ercole I d'Este 32, 44121, Ferrara, Italy aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10597-10609 0175-7598 99:24<10597 2015 99 253 https://doi.org/10.1007/s00253-015-6908-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6908-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sabia Alessandra Department of Life Sciences and Biotechnology, University of Ferrara, Corso Ercole I d'Este 32, 44121, Ferrara, Italy aut NATIONALLICENCE 700 1- Baldisserotto Costanza Department of Life Sciences and Biotechnology, University of Ferrara, Corso Ercole I d'Este 32, 44121, Ferrara, Italy aut NATIONALLICENCE 700 1- Biondi Stefania Department of Biological, Geological and Environmental Sciences, University of Bologna, via Irnerio 42, 40126, Bologna, Italy aut NATIONALLICENCE 700 1- Marchesini Roberta Department of Life Sciences and Biotechnology, University of Ferrara, Corso Ercole I d'Este 32, 44121, Ferrara, Italy aut NATIONALLICENCE 700 1- Tedeschi Paola Department of Chemical and Pharmaceutical Science, University of Ferrara, via Fossato di Mortara 17, 44121, Ferrara, Italy aut NATIONALLICENCE 700 1- Maietti Annalisa Department of Chemical and Pharmaceutical Science, University of Ferrara, via Fossato di Mortara 17, 44121, Ferrara, Italy aut NATIONALLICENCE 700 1- Giovanardi Martina Department of Life Sciences and Biotechnology, University of Ferrara, Corso Ercole I d'Este 32, 44121, Ferrara, Italy aut NATIONALLICENCE 700 1- Ferroni Lorenzo Department of Life Sciences and Biotechnology, University of Ferrara, Corso Ercole I d'Este 32, 44121, Ferrara, Italy aut NATIONALLICENCE 700 1- Pancaldi Simonetta Department of Life Sciences and Biotechnology, University of Ferrara, Corso Ercole I d'Este 32, 44121, Ferrara, Italy aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10597-10609 0175-7598 99:24<10597 2015 99 253