Unveiling PHA-storing populations using molecular methods
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| 008 | 210128e20151201xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-015-7010-6 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-7010-6 | ||
| 245 | 0 | 0 | |a Unveiling PHA-storing populations using molecular methods |h [Elektronische Daten] |c [Diogo Queirós, Paulo Lemos, Simona Rossetti, Luísa Serafim] |
| 520 | 3 | |a Enrichment of mixed microbial cultures (MMCs) in polyhydroxyalkanoate (PHA)-storing microorganisms must take place to develop a successful PHA production process. Moreover, throughout the operational period of a MMC system, the population needs to be checked in order to understand the changes in the performance that eventually occurred. For these reasons, it is necessary to monitor the population evolution, in order to identify the different groups of microorganisms and relate them with the storage capacity and kinetics of the MMC. Regarding this particular process, several culture-independent molecular techniques were already applied, with the use of hybridization techniques such fluorescence in situ hybridization and also PCR-based methods like denaturing gradient gel electrophoresis, terminal restriction fragment length polymorphism, pyrosequencing, and quantitative PCR standing out. This review intends, thus, to look at the molecular methods currently applied in monitoring the PHA-storing population evolution and how they can be combined with the evolutionary engineering step in order to optimize the overall process. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Mixed microbial culture |2 nationallicence | |
| 690 | 7 | |a Polyhydroxyalkanoates |2 nationallicence | |
| 690 | 7 | |a Ecology |2 nationallicence | |
| 690 | 7 | |a Hybridization |2 nationallicence | |
| 690 | 7 | |a PCR-based methods |2 nationallicence | |
| 700 | 1 | |a Queirós |D Diogo |u CICECO-Aveiro Institute of Materials, Chemistry Department, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal |4 aut | |
| 700 | 1 | |a Lemos |D Paulo |u LAQV, REQUIMTE, Chemistry Department, Faculty of Science and Technology, University Nova of Lisbon, 2829-516, Caparica, Portugal |4 aut | |
| 700 | 1 | |a Rossetti |D Simona |u Water Research Institute, C.N.R., Via Salaria Km 29,300, 00015, Monterotondo, Italy |4 aut | |
| 700 | 1 | |a Serafim |D Luísa |u CICECO-Aveiro Institute of Materials, Chemistry Department, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/24(2015-12-01), 10433-10446 |x 0175-7598 |q 99:24<10433 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-7010-6 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a review-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-7010-6 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Queirós |D Diogo |u CICECO-Aveiro Institute of Materials, Chemistry Department, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Lemos |D Paulo |u LAQV, REQUIMTE, Chemistry Department, Faculty of Science and Technology, University Nova of Lisbon, 2829-516, Caparica, Portugal |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Rossetti |D Simona |u Water Research Institute, C.N.R., Via Salaria Km 29,300, 00015, Monterotondo, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Serafim |D Luísa |u CICECO-Aveiro Institute of Materials, Chemistry Department, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/24(2015-12-01), 10433-10446 |x 0175-7598 |q 99:24<10433 |1 2015 |2 99 |o 253 | ||