caa a22 4500 605502536 CHVBK 20210128100606.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00253-015-7010-6 doi (NATIONALLICENCE)springer-10.1007/s00253-015-7010-6 Unveiling PHA-storing populations using molecular methods [Elektronische Daten] [Diogo Queirós, Paulo Lemos, Simona Rossetti, Luísa Serafim] Enrichment of mixed microbial cultures (MMCs) in polyhydroxyalkanoate (PHA)-storing microorganisms must take place to develop a successful PHA production process. Moreover, throughout the operational period of a MMC system, the population needs to be checked in order to understand the changes in the performance that eventually occurred. For these reasons, it is necessary to monitor the population evolution, in order to identify the different groups of microorganisms and relate them with the storage capacity and kinetics of the MMC. Regarding this particular process, several culture-independent molecular techniques were already applied, with the use of hybridization techniques such fluorescence in situ hybridization and also PCR-based methods like denaturing gradient gel electrophoresis, terminal restriction fragment length polymorphism, pyrosequencing, and quantitative PCR standing out. This review intends, thus, to look at the molecular methods currently applied in monitoring the PHA-storing population evolution and how they can be combined with the evolutionary engineering step in order to optimize the overall process. Springer-Verlag Berlin Heidelberg, 2015 Mixed microbial culture nationallicence Polyhydroxyalkanoates nationallicence Ecology nationallicence Hybridization nationallicence PCR-based methods nationallicence Queirós Diogo CICECO-Aveiro Institute of Materials, Chemistry Department, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal aut Lemos Paulo LAQV, REQUIMTE, Chemistry Department, Faculty of Science and Technology, University Nova of Lisbon, 2829-516, Caparica, Portugal aut Rossetti Simona Water Research Institute, C.N.R., Via Salaria Km 29,300, 00015, Monterotondo, Italy aut Serafim Luísa CICECO-Aveiro Institute of Materials, Chemistry Department, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10433-10446 0175-7598 99:24<10433 2015 99 253 https://doi.org/10.1007/s00253-015-7010-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-7010-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Queirós Diogo CICECO-Aveiro Institute of Materials, Chemistry Department, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal aut NATIONALLICENCE 700 1- Lemos Paulo LAQV, REQUIMTE, Chemistry Department, Faculty of Science and Technology, University Nova of Lisbon, 2829-516, Caparica, Portugal aut NATIONALLICENCE 700 1- Rossetti Simona Water Research Institute, C.N.R., Via Salaria Km 29,300, 00015, Monterotondo, Italy aut NATIONALLICENCE 700 1- Serafim Luísa CICECO-Aveiro Institute of Materials, Chemistry Department, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10433-10446 0175-7598 99:24<10433 2015 99 253