Metabolic engineering of Escherichia coli to improve recombinant protein production
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| 008 | 210128e20151201xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-015-6955-9 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6955-9 | ||
| 245 | 0 | 0 | |a Metabolic engineering of Escherichia coli to improve recombinant protein production |h [Elektronische Daten] |c [Min Liu, Xinjun Feng, Yamei Ding, Guang Zhao, Huizhou Liu, Mo Xian] |
| 520 | 3 | |a Escherichia coli is one of the most widely used strains for recombinant protein production. However, obstacles also exist in both academic researches and industrial applications, such as the metabolic burden, the carbon source waste, and the cells' physiological deterioration. This article reviews recent approaches for improving recombinant protein production in metabolic engineering, including workhorse selection, stress factor application, and carbon flux regulation. Selecting a suitable host is the first key point for recombinant protein production. In general, it all depends on characteristics of the strains and the target proteins. It will be triggered cells physiological deterioration when the medium is significantly different from the cell's natural environment. Coexpression of stress factors can help proteins to fold into their native conformation. Carbon flux regulation is a direct approach for redirecting more carbon flux toward the desirable pathways and products. However, some undesirable consequences are usually found in metabolic engineering, such as glucose transport inhibition, cell growth retardation, and useless metabolite accumulation. More efficient regulators and platform cell factories should be explored to meet a variety of production demands. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Recombinant protein production |2 nationallicence | |
| 690 | 7 | |a Metabolic engineering |2 nationallicence | |
| 690 | 7 | |a Workhorse selection |2 nationallicence | |
| 690 | 7 | |a Stress factors |2 nationallicence | |
| 690 | 7 | |a Carbon flux regulation |2 nationallicence | |
| 690 | 7 | |a Growth retardation |2 nationallicence | |
| 700 | 1 | |a Liu |D Min |u CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 266101, Qingdao, China |4 aut | |
| 700 | 1 | |a Feng |D Xinjun |u CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 266101, Qingdao, China |4 aut | |
| 700 | 1 | |a Ding |D Yamei |u Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China |4 aut | |
| 700 | 1 | |a Zhao |D Guang |u CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 266101, Qingdao, China |4 aut | |
| 700 | 1 | |a Liu |D Huizhou |u CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 266101, Qingdao, China |4 aut | |
| 700 | 1 | |a Xian |D Mo |u CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 266101, Qingdao, China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/24(2015-12-01), 10367-10377 |x 0175-7598 |q 99:24<10367 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6955-9 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a review-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6955-9 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Liu |D Min |u CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 266101, Qingdao, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Feng |D Xinjun |u CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 266101, Qingdao, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ding |D Yamei |u Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhao |D Guang |u CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 266101, Qingdao, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Liu |D Huizhou |u CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 266101, Qingdao, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Xian |D Mo |u CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 266101, Qingdao, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/24(2015-12-01), 10367-10377 |x 0175-7598 |q 99:24<10367 |1 2015 |2 99 |o 253 | ||