caa a22 4500 605502560 CHVBK 20210128100606.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00253-015-6955-9 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6955-9 Metabolic engineering of Escherichia coli to improve recombinant protein production [Elektronische Daten] [Min Liu, Xinjun Feng, Yamei Ding, Guang Zhao, Huizhou Liu, Mo Xian] Escherichia coli is one of the most widely used strains for recombinant protein production. However, obstacles also exist in both academic researches and industrial applications, such as the metabolic burden, the carbon source waste, and the cells' physiological deterioration. This article reviews recent approaches for improving recombinant protein production in metabolic engineering, including workhorse selection, stress factor application, and carbon flux regulation. Selecting a suitable host is the first key point for recombinant protein production. In general, it all depends on characteristics of the strains and the target proteins. It will be triggered cells physiological deterioration when the medium is significantly different from the cell's natural environment. Coexpression of stress factors can help proteins to fold into their native conformation. Carbon flux regulation is a direct approach for redirecting more carbon flux toward the desirable pathways and products. However, some undesirable consequences are usually found in metabolic engineering, such as glucose transport inhibition, cell growth retardation, and useless metabolite accumulation. More efficient regulators and platform cell factories should be explored to meet a variety of production demands. Springer-Verlag Berlin Heidelberg, 2015 Recombinant protein production nationallicence Metabolic engineering nationallicence Workhorse selection nationallicence Stress factors nationallicence Carbon flux regulation nationallicence Growth retardation nationallicence Liu Min CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 266101, Qingdao, China aut Feng Xinjun CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 266101, Qingdao, China aut Ding Yamei Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China aut Zhao Guang CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 266101, Qingdao, China aut Liu Huizhou CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 266101, Qingdao, China aut Xian Mo CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 266101, Qingdao, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10367-10377 0175-7598 99:24<10367 2015 99 253 https://doi.org/10.1007/s00253-015-6955-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6955-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Min CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 266101, Qingdao, China aut NATIONALLICENCE 700 1- Feng Xinjun CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 266101, Qingdao, China aut NATIONALLICENCE 700 1- Ding Yamei Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China aut NATIONALLICENCE 700 1- Zhao Guang CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 266101, Qingdao, China aut NATIONALLICENCE 700 1- Liu Huizhou CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 266101, Qingdao, China aut NATIONALLICENCE 700 1- Xian Mo CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 266101, Qingdao, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10367-10377 0175-7598 99:24<10367 2015 99 253