caa a22 4500 605502579 CHVBK 20210128100606.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00253-015-6940-3 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6940-3 Glutathione is involved in physiological response of Candida utilis to acid stress [Elektronische Daten] [Da-Hui Wang, Jun-Li Zhang, Ying-Ying Dong, Gong-Yuan Wei, Bin Qi] Candida utilis often encounters an acid stress environment when hexose and pentose are metabolized to produce acidic bio-based materials. In order to reveal the physiological role of glutathione (GSH) in the response of cells of this industrial yeast to acid stress, an efficient GSH-producing strain of C. utilis CCTCC M 209298 and its mutants deficient in GSH biosynthesis, C. utilis Δgsh1 and Δgsh2, were used in this study. A long-term mild acid challenge (pH3.5 for 6h) and a short-term severe acid challenge (pH1.5 for 2h) were conducted at 18h during batch culture of the yeast to generate acid stress conditions. Differences in the physiological performances among the three strains under acid stress were analyzed in terms of GSH biosynthesis and distribution; intracellular pH; activities of γ-glutamylcysteine synthetase, catalase, and superoxide dismutase; intracellular ATP level; and ATP/ADP ratio. The intracellular GSH content of the yeast was found to be correlated with changes in physiological data, and a higher intracellular GSH content led to greater relief of cells to the acid stress, suggesting that GSH may be involved in protecting C. utilis against acid stress. Results presented in this manuscript not only increase our understanding of the impact of GSH on the physiology of C. utilis but also help us to comprehend the mechanism underlying the response to acid stress of eukaryotic microorganisms. Springer-Verlag Berlin Heidelberg, 2015 Glutathione nationallicence Candida utilis nationallicence Acid stress nationallicence Intracellular pH nationallicence ATP nationallicence γ -Glutamylcysteine synthetase nationallicence Wang Da-Hui School of Biology and Basic Medical Sciences, Soochow University, 215123, Suzhou, People's Republic of China aut Zhang Jun-Li School of Biology and Basic Medical Sciences, Soochow University, 215123, Suzhou, People's Republic of China aut Dong Ying-Ying School of Biology and Basic Medical Sciences, Soochow University, 215123, Suzhou, People's Republic of China aut Wei Gong-Yuan School of Biology and Basic Medical Sciences, Soochow University, 215123, Suzhou, People's Republic of China aut Qi Bin School of Biotechnology and Food Engineering, Changshu Institute of Technology, 215500, Changshu, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10669-10679 0175-7598 99:24<10669 2015 99 253 https://doi.org/10.1007/s00253-015-6940-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6940-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Da-Hui School of Biology and Basic Medical Sciences, Soochow University, 215123, Suzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Jun-Li School of Biology and Basic Medical Sciences, Soochow University, 215123, Suzhou, People's Republic of China aut NATIONALLICENCE 700 1- Dong Ying-Ying School of Biology and Basic Medical Sciences, Soochow University, 215123, Suzhou, People's Republic of China aut NATIONALLICENCE 700 1- Wei Gong-Yuan School of Biology and Basic Medical Sciences, Soochow University, 215123, Suzhou, People's Republic of China aut NATIONALLICENCE 700 1- Qi Bin School of Biotechnology and Food Engineering, Changshu Institute of Technology, 215500, Changshu, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10669-10679 0175-7598 99:24<10669 2015 99 253