Microbial community in a hydrogenotrophic denitrification reactor based on pyrosequencing
Gespeichert in:
Verfasser / Beitragende:
[Hongyu Wang, Qiulai He, Dan Chen, Li Wei, Zhuocheng Zou, Jun Zhou, Kai Yang, Huining Zhang]
Ort, Verlag, Jahr:
2015
Enthalten in:
Applied Microbiology and Biotechnology, 99/24(2015-12-01), 10829-10837
Format:
Artikel (online)
Online Zugang:
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6929-y |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6929-y | ||
| 245 | 0 | 0 | |a Microbial community in a hydrogenotrophic denitrification reactor based on pyrosequencing |h [Elektronische Daten] |c [Hongyu Wang, Qiulai He, Dan Chen, Li Wei, Zhuocheng Zou, Jun Zhou, Kai Yang, Huining Zhang] |
| 520 | 3 | |a Effects of some key factors on hydrogenotrophic denitrification were investigated in lab-scale bioreactors. Results indicated that optimum biomass loading, pH value, temperature, nitrate loading, and C/N ratio in this study were optical density at 600nm (OD600) of 0.173, 6.0∼7.0, 35°C, 105mgL−1, and 30, respectively. To obtain a better understanding of microbial community in the bioreactors, the 454-pyrosequencing technology was used to characterize the 16S ribosomal RNA (16S rRNA) gene of bacteria in selected samples Y1 and Y2, in which a total of 62,559 effective sequences (36,445 in Y1 and 26,114 in Y2) were obtained. The taxonomic complexities in two samples were compared at phylum/class/genus levels. In total, 41 bacterial phyla, 43 bacterial classes, and 312 genera were detected, where phylum Firmicutes, class Clostridia were most abundant. Genus Proteiniclasticum was predominant among the top 100 genera. This work aims to add some novel insights into hydrogenotrophic denitrification process and its microbial community structures in bioreactors. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Hydrogenotrophic denitrification |2 nationallicence | |
| 690 | 7 | |a Factors |2 nationallicence | |
| 690 | 7 | |a Pyrosequencing |2 nationallicence | |
| 690 | 7 | |a Microbial community |2 nationallicence | |
| 690 | 7 | |a Firmicutes |2 nationallicence | |
| 700 | 1 | |a Wang |D Hongyu |u School of Civil Engineering, Wuhan University, 430072, Wuhan, China |4 aut | |
| 700 | 1 | |a He |D Qiulai |u School of Civil Engineering, Wuhan University, 430072, Wuhan, China |4 aut | |
| 700 | 1 | |a Chen |D Dan |u School of Civil Engineering, Wuhan University, 430072, Wuhan, China |4 aut | |
| 700 | 1 | |a Wei |D Li |u State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, 150090, Harbin, China |4 aut | |
| 700 | 1 | |a Zou |D Zhuocheng |u School of Civil Engineering, Wuhan University, 430072, Wuhan, China |4 aut | |
| 700 | 1 | |a Zhou |D Jun |u School of Civil Engineering, Wuhan University, 430072, Wuhan, China |4 aut | |
| 700 | 1 | |a Yang |D Kai |u School of Civil Engineering, Wuhan University, 430072, Wuhan, China |4 aut | |
| 700 | 1 | |a Zhang |D Huining |u School of Civil Engineering, Wuhan University, 430072, Wuhan, China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/24(2015-12-01), 10829-10837 |x 0175-7598 |q 99:24<10829 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6929-y |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6929-y |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wang |D Hongyu |u School of Civil Engineering, Wuhan University, 430072, Wuhan, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a He |D Qiulai |u School of Civil Engineering, Wuhan University, 430072, Wuhan, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Chen |D Dan |u School of Civil Engineering, Wuhan University, 430072, Wuhan, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wei |D Li |u State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, 150090, Harbin, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zou |D Zhuocheng |u School of Civil Engineering, Wuhan University, 430072, Wuhan, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhou |D Jun |u School of Civil Engineering, Wuhan University, 430072, Wuhan, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yang |D Kai |u School of Civil Engineering, Wuhan University, 430072, Wuhan, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhang |D Huining |u School of Civil Engineering, Wuhan University, 430072, Wuhan, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/24(2015-12-01), 10829-10837 |x 0175-7598 |q 99:24<10829 |1 2015 |2 99 |o 253 | ||