caa a22 4500 605502617 CHVBK 20210128100606.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00253-015-6929-y doi (NATIONALLICENCE)springer-10.1007/s00253-015-6929-y Microbial community in a hydrogenotrophic denitrification reactor based on pyrosequencing [Elektronische Daten] [Hongyu Wang, Qiulai He, Dan Chen, Li Wei, Zhuocheng Zou, Jun Zhou, Kai Yang, Huining Zhang] Effects of some key factors on hydrogenotrophic denitrification were investigated in lab-scale bioreactors. Results indicated that optimum biomass loading, pH value, temperature, nitrate loading, and C/N ratio in this study were optical density at 600nm (OD600) of 0.173, 6.0∼7.0, 35°C, 105mgL−1, and 30, respectively. To obtain a better understanding of microbial community in the bioreactors, the 454-pyrosequencing technology was used to characterize the 16S ribosomal RNA (16S rRNA) gene of bacteria in selected samples Y1 and Y2, in which a total of 62,559 effective sequences (36,445 in Y1 and 26,114 in Y2) were obtained. The taxonomic complexities in two samples were compared at phylum/class/genus levels. In total, 41 bacterial phyla, 43 bacterial classes, and 312 genera were detected, where phylum Firmicutes, class Clostridia were most abundant. Genus Proteiniclasticum was predominant among the top 100 genera. This work aims to add some novel insights into hydrogenotrophic denitrification process and its microbial community structures in bioreactors. Springer-Verlag Berlin Heidelberg, 2015 Hydrogenotrophic denitrification nationallicence Factors nationallicence Pyrosequencing nationallicence Microbial community nationallicence Firmicutes nationallicence Wang Hongyu School of Civil Engineering, Wuhan University, 430072, Wuhan, China aut He Qiulai School of Civil Engineering, Wuhan University, 430072, Wuhan, China aut Chen Dan School of Civil Engineering, Wuhan University, 430072, Wuhan, China aut Wei Li State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, 150090, Harbin, China aut Zou Zhuocheng School of Civil Engineering, Wuhan University, 430072, Wuhan, China aut Zhou Jun School of Civil Engineering, Wuhan University, 430072, Wuhan, China aut Yang Kai School of Civil Engineering, Wuhan University, 430072, Wuhan, China aut Zhang Huining School of Civil Engineering, Wuhan University, 430072, Wuhan, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10829-10837 0175-7598 99:24<10829 2015 99 253 https://doi.org/10.1007/s00253-015-6929-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6929-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Hongyu School of Civil Engineering, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- He Qiulai School of Civil Engineering, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Chen Dan School of Civil Engineering, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Wei Li State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, 150090, Harbin, China aut NATIONALLICENCE 700 1- Zou Zhuocheng School of Civil Engineering, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Zhou Jun School of Civil Engineering, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Yang Kai School of Civil Engineering, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Zhang Huining School of Civil Engineering, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10829-10837 0175-7598 99:24<10829 2015 99 253