caa a22 4500 605502692 CHVBK 20210128100607.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00253-015-6924-3 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6924-3 Development of the dichlorvos-ammonia (DV-AM) method for the visual detection of aflatoxigenic fungi [Elektronische Daten] [Kimiko Yabe, Hidemi Hatabayashi, Akifumi Ikehata, Yazhi Zheng, Masayo Kushiro] Aflatoxins (AFs) are carcinogenic and toxic secondary metabolites produced mainly by Aspergillus flavus and Aspergillus parasiticus. To monitor and regulate the AF contamination of crops, a sensitive and precise detection method for these toxigenic fungi in environments is necessary. We herein developed a novel visual detection method, the dichlorvos-ammonia (DV-AM) method, for identifying AF-producing fungi using DV and AM vapor on agar culture plates, in which DV inhibits the esterase in AF biosynthesis, causing the accumulation of anthraquinone precursors (versiconal hemiacetal acetate and versiconol acetate) of AFs in mycelia on the agar plate, followed by a change in the color of the colonies from light yellow to brilliant purple-red by the AM vapor treatment. We also investigated the appropriate culture conditions to increase the color intensity. It should be noted that other species producing the same precursors of AFs such as Aspergillus nidulans and Aspergillus versicolor could be discriminated from the Aspergillus section Flavi based on the differences of their phenotypes. The DV-AM method was also useful for the isolation of nonaflatoxigenic fungi showing no color change, for screening microorganisms that inhibit the AF production by fungi, and for the characterization of the fungi infecting corn kernels. Thus, the DV-AM method can provide a highly sensitive and visible indicator for the detection of aflatoxigenic fungi. Springer-Verlag Berlin Heidelberg, 2015 Visible detection nationallicence Dichlorvos nationallicence Ammonia vapor nationallicence Fungi nationallicence Aflatoxin nationallicence Yabe Kimiko National Food Research Institute, National Agriculture and Food Research Organization (NARO), 305-8642, Tsukuba, Ibaraki, Japan aut Hatabayashi Hidemi National Food Research Institute, National Agriculture and Food Research Organization (NARO), 305-8642, Tsukuba, Ibaraki, Japan aut Ikehata Akifumi National Food Research Institute, National Agriculture and Food Research Organization (NARO), 305-8642, Tsukuba, Ibaraki, Japan aut Zheng Yazhi National Food Research Institute, National Agriculture and Food Research Organization (NARO), 305-8642, Tsukuba, Ibaraki, Japan aut Kushiro Masayo National Food Research Institute, National Agriculture and Food Research Organization (NARO), 305-8642, Tsukuba, Ibaraki, Japan aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10681-10694 0175-7598 99:24<10681 2015 99 253 https://doi.org/10.1007/s00253-015-6924-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6924-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yabe Kimiko National Food Research Institute, National Agriculture and Food Research Organization (NARO), 305-8642, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Hatabayashi Hidemi National Food Research Institute, National Agriculture and Food Research Organization (NARO), 305-8642, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Ikehata Akifumi National Food Research Institute, National Agriculture and Food Research Organization (NARO), 305-8642, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Zheng Yazhi National Food Research Institute, National Agriculture and Food Research Organization (NARO), 305-8642, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Kushiro Masayo National Food Research Institute, National Agriculture and Food Research Organization (NARO), 305-8642, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10681-10694 0175-7598 99:24<10681 2015 99 253