Antimicrobial potentials of Helicteres isora silver nanoparticles against extensively drug-resistant (XDR) clinical isolates of Pseudomonas aeruginosa
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6938-x |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6938-x | ||
| 245 | 0 | 0 | |a Antimicrobial potentials of Helicteres isora silver nanoparticles against extensively drug-resistant (XDR) clinical isolates of Pseudomonas aeruginosa |h [Elektronische Daten] |c [Nikunj Mapara, Mansi Sharma, Varsha Shriram, Renu Bharadwaj, K. Mohite, Vinay Kumar] |
| 520 | 3 | |a Pseudomonas aeruginosa is a leading opportunistic pathogen and its expanding drug resistance is a growing menace to public health. Its ubiquitous nature and multiple resistance mechanisms make it a difficult target for antimicrobial chemotherapy and require a fresh approach for developing new antimicrobial agents against it. The broad-spectrum antibacterial effects of silver nanoparticles (SNPs) make them an excellent candidate for use in the medical field. However, attempts made to check their potency against extensively drug-resistant (XDR) microbes are meager. This study describes the biosynthesis and biostabilization of SNPs by Helicteres isora aqueous fruit extract and their characterization by ultraviolet-visible spectroscopy, transmission electron microscopy, dynamic light scattering, X-ray diffraction, and Fourier transform infrared spectroscopy. Majority of SNPs synthesized were of 8--20-nm size. SNPs exhibited dose-dependent antibacterial activities against four XDR P. aeruginosa (XDR-PA) clinical isolates as revealed by growth curves, with a minimum inhibitory concentration of 300μg/ml. The SNPs exhibited antimicrobial activity against all strains, with maximum zone of inhibition (16.4mm) in XRD-PA-2 at 1000μg/ml. Amongst four strains, their susceptibilities to SNPs were in the following order: XDR-PA-2>XDR-PA-4>XDR-PA-3>XDR-PA-1. The exposure of bacterial cells to 300μg/ml SNPs resulted into a substantial leakage of reducing sugars and proteins, inactivation of respiratory chain dehydrogenases, and eventual cell death. SNPs also induced lipid peroxidation, a possible underlying factor to membrane porosity. The effects were more pronounced in XDR-PA-2 which may be correlated with its higher susceptibility to SNPs. These results are indicative of SNP-induced turbulence of membranous permeability as an important causal factor in XDR-PA growth inhibition and death. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Extensively drug resistant (XDR) |2 nationallicence | |
| 690 | 7 | |a Pseudomonas aeruginosa |2 nationallicence | |
| 690 | 7 | |a Helicteres isora |2 nationallicence | |
| 690 | 7 | |a Silver nanoparticles |2 nationallicence | |
| 690 | 7 | |a Antimicrobial agents |2 nationallicence | |
| 690 | 7 | |a Bacterial membrane |2 nationallicence | |
| 700 | 1 | |a Mapara |D Nikunj |u Department of Biotechnology, Modern College of Arts, Science and Commerce, S. P. Pune University, Ganeshkhind, 411 016, Pune, India |4 aut | |
| 700 | 1 | |a Sharma |D Mansi |u Department of Biotechnology, Modern College of Arts, Science and Commerce, S. P. Pune University, Ganeshkhind, 411 016, Pune, India |4 aut | |
| 700 | 1 | |a Shriram |D Varsha |u Department of Botany, Prof. Ramkrishna More Arts, Commerce and Science College, S. P. Pune University, Akurdi, 411044, Pune, India |4 aut | |
| 700 | 1 | |a Bharadwaj |D Renu |u Department of Microbiology, B. J. Government Medical College, 411001, Pune, India |4 aut | |
| 700 | 1 | |a Mohite |D K. |u School of Energy Studies, Department of Physics, Savitribai Phule Pune University, Ganeshkhind, 411007, Pune, India |4 aut | |
| 700 | 1 | |a Kumar |D Vinay |u Department of Biotechnology, Modern College of Arts, Science and Commerce, S. P. Pune University, Ganeshkhind, 411 016, Pune, India |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/24(2015-12-01), 10655-10667 |x 0175-7598 |q 99:24<10655 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6938-x |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6938-x |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Mapara |D Nikunj |u Department of Biotechnology, Modern College of Arts, Science and Commerce, S. P. Pune University, Ganeshkhind, 411 016, Pune, India |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sharma |D Mansi |u Department of Biotechnology, Modern College of Arts, Science and Commerce, S. P. Pune University, Ganeshkhind, 411 016, Pune, India |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Shriram |D Varsha |u Department of Botany, Prof. Ramkrishna More Arts, Commerce and Science College, S. P. Pune University, Akurdi, 411044, Pune, India |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Bharadwaj |D Renu |u Department of Microbiology, B. J. Government Medical College, 411001, Pune, India |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Mohite |D K. |u School of Energy Studies, Department of Physics, Savitribai Phule Pune University, Ganeshkhind, 411007, Pune, India |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kumar |D Vinay |u Department of Biotechnology, Modern College of Arts, Science and Commerce, S. P. Pune University, Ganeshkhind, 411 016, Pune, India |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/24(2015-12-01), 10655-10667 |x 0175-7598 |q 99:24<10655 |1 2015 |2 99 |o 253 | ||