caa a22 4500 605502714 CHVBK 20210128100607.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00253-015-6918-1 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6918-1 Isolation of AHL-degrading bacteria from micro-algal cultures and their impact on algal growth and on virulence of Vibrio campbellii to prawn larvae [Elektronische Daten] [Gde Pande, Fatin Natrah, Ace Flandez, Uday Kumar, Yufeng Niu, Peter Bossier, Tom Defoirdt] Inactivation of quorum sensing (QS) signal molecules, such as acylhomoserine lactones (AHLs) of pathogenic bacteria, has been proposed as a novel method to combat bacterial diseases in aquaculture. Despite the importance of micro-algae for aquaculture, AHL degradation by bacteria associated with micro-algal cultures has thus far not been investigated. In this study, we isolated Pseudomonas sp. NFMI-T and Bacillus sp. NFMI-C from open cultures of the micro-algae Tetraselmis suecica and Chaetoceros muelleri, respectively. An AHL degradation assay showed that either monocultures or co-cultures of the isolates were able to degrade the AHL N-hexanoyl-l-homoserine lactone. In contrast, only Bacillus sp. NFMI-C was able to inactivate N-hydroxybutanoyl-l-homoserine lactone, the AHL produced by Vibrio campbellii. The isolated bacteria were able to persist for up to 3weeks in conventionalized micro-algal cultures, indicating that they were able to establish and maintain themselves within open algal cultures. Using gnotobiotic algal cultures, we found that the isolates did not affect growth of the micro-algae from which they were isolated, whereas a mixture of both isolates increased the growth of Tetraselmis and decreased the growth of Chaetoceros. Finally, addition of Bacillus sp. NFMI-C to the rearing water of giant river prawn (Macrobrachium rosenbergii) larvae significantly improved survival of the larvae when challenged with pathogenic V. campbellii, whereas it had no effect on larval growth. Springer-Verlag Berlin Heidelberg, 2015 Quorum quenching nationallicence Acylase nationallicence Lactonase nationallicence Micro-algae nationallicence Macrobrachium rosenbergii nationallicence Vibriosis nationallicence Pande Gde Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Rozier 44, B-9000, Ghent, Belgium aut Natrah Fatin Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Malaysia aut Flandez Ace Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Rozier 44, B-9000, Ghent, Belgium aut Kumar Uday Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Rozier 44, B-9000, Ghent, Belgium aut Niu Yufeng Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Rozier 44, B-9000, Ghent, Belgium aut Bossier Peter Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Rozier 44, B-9000, Ghent, Belgium aut Defoirdt Tom Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Rozier 44, B-9000, Ghent, Belgium aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10805-10813 0175-7598 99:24<10805 2015 99 253 https://doi.org/10.1007/s00253-015-6918-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6918-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pande Gde Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Rozier 44, B-9000, Ghent, Belgium aut NATIONALLICENCE 700 1- Natrah Fatin Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Malaysia aut NATIONALLICENCE 700 1- Flandez Ace Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Rozier 44, B-9000, Ghent, Belgium aut NATIONALLICENCE 700 1- Kumar Uday Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Rozier 44, B-9000, Ghent, Belgium aut NATIONALLICENCE 700 1- Niu Yufeng Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Rozier 44, B-9000, Ghent, Belgium aut NATIONALLICENCE 700 1- Bossier Peter Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Rozier 44, B-9000, Ghent, Belgium aut NATIONALLICENCE 700 1- Defoirdt Tom Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Rozier 44, B-9000, Ghent, Belgium aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10805-10813 0175-7598 99:24<10805 2015 99 253