caa a22 4500 605502722 CHVBK 20210128100607.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00253-015-6919-0 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6919-0 Evaluation of soil bioremediation techniques in an aged diesel spill at the Antarctic Peninsula [Elektronische Daten] [Hugo de Jesus, Raquel Peixoto, Juliano Cury, Jan van Elsas, Alexandre Rosado] Many areas on the Antarctic continent already suffer from the direct and indirect influences of human activities. The main cause of contamination is petroleum hydrocarbons because this compound is used as a source of energy at the many research stations around the continent. Thus, the current study aims to evaluate treatments for bioremediation (biostimulation, bioaugmentation, and bioaugmentation + biostimulation) using soils from around the Brazilian Antarctic Station "Comandante Ferraz” (EACF), King George Island, Antarctic Peninsula. The experiment lasted for 45days, and at the end of this period, chemical and molecular analyses were performed. Those analyses included the quantification of carbon and nitrogen, denaturing gradient gel electrophoresis (DGGE) analysis (with gradient denaturation), real-time PCR, and quantification of total hydrocarbons and polyaromatics. Molecular tests evaluated changes in the profile and quantity of the rrs genes of archaea and bacteria and also the alkB gene. The influence of the treatments tested was directly related to the type of soil used. The work confirmed that despite the extreme conditions found in Antarctic soils, the bacterial strains degraded hydrocarbons and bioremediation treatments directly influenced the microbial communities present in these soils even in short periods. Although the majority of the previous studies demonstrate that the addition of fertilizer seems to be most effective at promoting bioremediation, our results show that for some conditions, autochthonous bioaugmentation (ABA) treatment is indicated. This work highlights the importance of understanding the processes of recovery of contaminated environments in polar regions because time is crucial to the soil recovery and to choosing the appropriate treatment. Springer-Verlag Berlin Heidelberg, 2015 Soil nationallicence Bioremediation nationallicence Antarctica nationallicence Diesel nationallicence de Jesus Hugo LEMM—Laboratório de Ecologia Microbiana Molecular-Instituto de Microbiologia Paulo de Góes (IMPG), Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil aut Peixoto Raquel LEMM—Laboratório de Ecologia Microbiana Molecular-Instituto de Microbiologia Paulo de Góes (IMPG), Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil aut Cury Juliano Molecular Microbiology Laboratory, CSL/Universidade Federal de São João Del Rei, Sete Lagoas, Minas Gerais, Brazil aut van Elsas Jan Microbial Ecology Department, University of Groningen, Groningen, Netherlands aut Rosado Alexandre LEMM—Laboratório de Ecologia Microbiana Molecular-Instituto de Microbiologia Paulo de Góes (IMPG), Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10815-10827 0175-7598 99:24<10815 2015 99 253 https://doi.org/10.1007/s00253-015-6919-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6919-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- de Jesus Hugo LEMM—Laboratório de Ecologia Microbiana Molecular-Instituto de Microbiologia Paulo de Góes (IMPG), Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil aut NATIONALLICENCE 700 1- Peixoto Raquel LEMM—Laboratório de Ecologia Microbiana Molecular-Instituto de Microbiologia Paulo de Góes (IMPG), Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil aut NATIONALLICENCE 700 1- Cury Juliano Molecular Microbiology Laboratory, CSL/Universidade Federal de São João Del Rei, Sete Lagoas, Minas Gerais, Brazil aut NATIONALLICENCE 700 1- van Elsas Jan Microbial Ecology Department, University of Groningen, Groningen, Netherlands aut NATIONALLICENCE 700 1- Rosado Alexandre LEMM—Laboratório de Ecologia Microbiana Molecular-Instituto de Microbiologia Paulo de Góes (IMPG), Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10815-10827 0175-7598 99:24<10815 2015 99 253