caa a22 4500 605502749 CHVBK 20210128100607.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00253-015-6935-0 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6935-0 Sugarcane bagasse as support for immobilization of Bacillus pumilus HZ-2 and its use in bioremediation of mesotrione-contaminated soils [Elektronische Daten] [Jie Liu, Shaohua Chen, Jie Ding, Ying Xiao, Haitao Han, Guohua Zhong] The degrading microorganisms isolated from environment usually fail to degrade pollutants when used for bioremediation of contaminated soils; thus, additional treatments are needed to enhance biodegradation. In the present study, the potential of sugarcane bagasse as bacteria-immobilizing support was investigated in mesotrione biodegradation. A novel isolate Bacillus pumilus HZ-2 was applied in bacterial immobilization, which was capable of degrading over 95% of mesotrione at initial concentrations ranging from 25 to 200mgL−1 within 4days in flask-shaking tests. Scanning electron microscope (SEM) images showed that the bacterial cells were strongly absorbed and fully dispersed on bagasse surface after immobilization. Specially, 86.5 and 82.9% of mesotrione was eliminated by bacteria immobilized on bagasse of 100 and 60mesh, respectively, which indicated that this immobilization was able to maintain a high degrading activity of the bacteria. Analysis of the degradation products determined 2-amino-4-methylsulfonylbenzoic acid (AMBA) and 4-methylsulfonyl-2-nitrobenzoic acid (MNBA) as the main metabolites in the biodegradation pathway of mesotrione. In the sterile soil, approximately 90% of mesotrione was degraded after supplementing 5.0% of molasses in bacteria-bagasse composite, which greatly enhanced microbial adaptability and growth in the soil environment. In the field tests, over 75% of mesotrione in soil was degraded within 14days. The immobilized preparation demonstrated that mesotrione could be degraded at a wide range of pH values (5.0-8.0) and temperatures (25-35°C), especially at low concentrations of mesotrione (5 to 20mgkg−1). These results showed that sugarcane bagasse might be a good candidate as bacteria-immobilizing support to enhance mesotrione degradation by Bacillus p. HZ-2 in contaminated soils. Springer-Verlag Berlin Heidelberg, 2015 Sugarcane bagasse nationallicence Immobilization nationallicence Mesotrione nationallicence Metabolites nationallicence Bioremediation nationallicence Liu Jie Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, and Lab of Insect Toxicology, South China Agricultural University, 510642, Guangzhou, People's Republic of China aut Chen Shaohua Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, 510642, Guangzhou, People's Republic of China aut Ding Jie Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, and Lab of Insect Toxicology, South China Agricultural University, 510642, Guangzhou, People's Republic of China aut Xiao Ying Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, and Lab of Insect Toxicology, South China Agricultural University, 510642, Guangzhou, People's Republic of China aut Han Haitao Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, and Lab of Insect Toxicology, South China Agricultural University, 510642, Guangzhou, People's Republic of China aut Zhong Guohua Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, and Lab of Insect Toxicology, South China Agricultural University, 510642, Guangzhou, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10839-10851 0175-7598 99:24<10839 2015 99 253 https://doi.org/10.1007/s00253-015-6935-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6935-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Jie Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, and Lab of Insect Toxicology, South China Agricultural University, 510642, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Chen Shaohua Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, 510642, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Ding Jie Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, and Lab of Insect Toxicology, South China Agricultural University, 510642, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Xiao Ying Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, and Lab of Insect Toxicology, South China Agricultural University, 510642, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Han Haitao Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, and Lab of Insect Toxicology, South China Agricultural University, 510642, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhong Guohua Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, and Lab of Insect Toxicology, South China Agricultural University, 510642, Guangzhou, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/24(2015-12-01), 10839-10851 0175-7598 99:24<10839 2015 99 253