caa a22 4500 605502765 CHVBK 20210128100607.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00253-015-6768-x doi (NATIONALLICENCE)springer-10.1007/s00253-015-6768-x Proteomic analysis of conidia germination in Fusarium oxysporum f. sp. cubense tropical race 4 reveals new targets in ergosterol biosynthesis pathway for controlling Fusarium wilt of banana [Elektronische Daten] [Gui-Ming Deng, Qiao-Song Yang, Wei-Di He, Chun-Yu Li, Jing Yang, Cun-Wu Zuo, Jie Gao, Ou Sheng, Shao-Yun Lu, Sheng Zhang, Gan-Jun Yi] Conidial germination is a crucial step of the soilborne fungus Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4), a most important lethal disease of banana. In this study, a total of 3659 proteins were identified by isobaric tags for relative and absolute quantitation (iTRAQ)-based comparative proteomic approach, of which 1009 were differentially expressed during conidial germination of the fungus at 0, 3, 7, and 11h. Functional classification and bioinformatics analysis revealed that the majority of the differentially expressed proteins are involved in six metabolic pathways. Particularly, all differential proteins involved in the ergosterol biosynthesis pathway were significantly upregulated, indicating the importance of the ergosterol biosynthesis pathway to the conidial germination of Foc TR4. Quantitative RT-PCR, western blotting, and in vitro growth inhibition assay by several categories of fungicides on the Foc TR4 were used to validate the proteomics results. Four enzymes, C-24 sterol methyltransferase (ERG6), cytochrome P450 lanosterol C-14α-demethylase (EGR11), hydroxymethylglutaryl-CoA synthase (ERG13), and C-4 sterol methyl oxidase (ERG25), in the ergosterol biosynthesis pathway were identified and verified, and they hold great promise as new targets for effective inhibition of Foc TR4 early growth in controlling Fusarium wilt of banana. To the best of our knowledge, this report represents the first comprehensive study on proteomics profiling of conidia germination in Foc TR4. It provides new insights into a better understanding of the developmental processes of Foc TR4 spores. More importantly, by host plant-induced gene silencing (HIGS) technology, the new targets reported in this work allow us to develop novel transgenic banana leading to high protection from Fusarium wilt and to explore more effective antifungal drugs against either individual or multiple target proteins of Foc TR4. Springer-Verlag Berlin Heidelberg, 2015 Fusarium oxysporum f. sp. cubense tropical race 4 nationallicence Conidia germination nationallicence Banana nationallicence Quantitative proteomics nationallicence Ergosterol biosynthesis nationallicence Deng Gui-Ming Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, 510640, Guangzhou, China aut Yang Qiao-Song Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, 510640, Guangzhou, China aut He Wei-Di Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, 510640, Guangzhou, China aut Li Chun-Yu Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, 510640, Guangzhou, China aut Yang Jing Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, 510640, Guangzhou, China aut Zuo Cun-Wu Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, 510640, Guangzhou, China aut Gao Jie Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, 510640, Guangzhou, China aut Sheng Ou Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, 510640, Guangzhou, China aut Lu Shao-Yun College of Life Sciences, South China Agricultural University, 510640, Guangzhou, China aut Zhang Sheng Institute of Biotechnology, Cornell University, 14853-2703, Ithaca, NY, USA aut Yi Gan-Jun Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, 510640, Guangzhou, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/17(2015-09-01), 7189-7207 0175-7598 99:17<7189 2015 99 253 https://doi.org/10.1007/s00253-015-6768-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6768-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Deng Gui-Ming Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, 510640, Guangzhou, China aut NATIONALLICENCE 700 1- Yang Qiao-Song Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, 510640, Guangzhou, China aut NATIONALLICENCE 700 1- He Wei-Di Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, 510640, Guangzhou, China aut NATIONALLICENCE 700 1- Li Chun-Yu Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, 510640, Guangzhou, China aut NATIONALLICENCE 700 1- Yang Jing Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, 510640, Guangzhou, China aut NATIONALLICENCE 700 1- Zuo Cun-Wu Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, 510640, Guangzhou, China aut NATIONALLICENCE 700 1- Gao Jie Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, 510640, Guangzhou, China aut NATIONALLICENCE 700 1- Sheng Ou Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, 510640, Guangzhou, China aut NATIONALLICENCE 700 1- Lu Shao-Yun College of Life Sciences, South China Agricultural University, 510640, Guangzhou, China aut NATIONALLICENCE 700 1- Zhang Sheng Institute of Biotechnology, Cornell University, 14853-2703, Ithaca, NY, USA aut NATIONALLICENCE 700 1- Yi Gan-Jun Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, 510640, Guangzhou, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/17(2015-09-01), 7189-7207 0175-7598 99:17<7189 2015 99 253