caa a22 4500 605502811 CHVBK 20210128100607.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00253-015-6752-5 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6752-5 Microbial lipid production by oleaginous Rhodococci cultured in lignocellulosic autohydrolysates [Elektronische Daten] [Zhen Wei, Guangming Zeng, Fang Huang, Matyas Kosa, Qining Sun, Xianzhi Meng, Danlian Huang, Arthur Ragauskas] Metabolic synthesis of single cell oils (SCOs) for biodiesel application by heterotrophic oleaginous microorganisms is being hampered by the high cost of culture media. This study investigated the possibility of using loblolly pine and sweetgum autohydrolysates as economic feedstocks for microbial lipid production by oleaginous Rhodococcus opacus (R. opacus) PD630 and DSM 1069. Results revealed that when the substrates were detoxified by the removal of inhibitors (such as HMF—hydroxymethyl-furfural), the two strains exhibited viable growth patterns after a short adaptation/lag phase. R. opacus PD630 accumulated as much as 28.6% of its cell dry weight (CDW) in lipids while growing on detoxified sweetgum autohydrolysate (DSAH) that translates to 0.25g/l lipid yield. The accumulation of SCOs reached the level of oleagenicity in DSM 1069 cells (28.3% of CDW) as well, while being cultured on detoxified pine autohydrolysate (DPAH), with the maximum lipid yield of 0.31g/l. The composition of the obtained microbial oils varied depending on the substrates provided. These results indicate that lignocellulosic autohydrolysates can be used as low-cost fermentation substrates for microbial lipid production by wild-type R. opacus species. Consequently, the variety of applications for aqueous liquors from lignocellulosic pretreatment has been expanded, allowing for the further optimization of the integrated biorefinery. Springer-Verlag Berlin Heidelberg, 2015 Autohydrolysis nationallicence Hardwood nationallicence Softwood nationallicence Fatty acid methyl ester nationallicence Oleaginous nationallicence Rhodococcus nationallicence Fermentation nationallicence Wei Zhen College of Environmental Science and Engineering, Hunan University, 410082, Changsha, People's Republic of China aut Zeng Guangming College of Environmental Science and Engineering, Hunan University, 410082, Changsha, People's Republic of China aut Huang Fang School of Chemistry and Biochemistry, and Renewable Bioproducts Institute,, Georgia Institute of Technology,, 500 10th street N.W., 30332-0620, Atlanta, GA, USA aut Kosa Matyas School of Chemistry and Biochemistry, and Renewable Bioproducts Institute,, Georgia Institute of Technology,, 500 10th street N.W., 30332-0620, Atlanta, GA, USA aut Sun Qining School of Chemistry and Biochemistry, and Renewable Bioproducts Institute,, Georgia Institute of Technology,, 500 10th street N.W., 30332-0620, Atlanta, GA, USA aut Meng Xianzhi School of Chemistry and Biochemistry, and Renewable Bioproducts Institute,, Georgia Institute of Technology,, 500 10th street N.W., 30332-0620, Atlanta, GA, USA aut Huang Danlian College of Environmental Science and Engineering, Hunan University, 410082, Changsha, People's Republic of China aut Ragauskas Arthur School of Chemistry and Biochemistry, and Renewable Bioproducts Institute,, Georgia Institute of Technology,, 500 10th street N.W., 30332-0620, Atlanta, GA, USA aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/17(2015-09-01), 7369-7377 0175-7598 99:17<7369 2015 99 253 https://doi.org/10.1007/s00253-015-6752-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6752-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wei Zhen College of Environmental Science and Engineering, Hunan University, 410082, Changsha, People's Republic of China aut NATIONALLICENCE 700 1- Zeng Guangming College of Environmental Science and Engineering, Hunan University, 410082, Changsha, People's Republic of China aut NATIONALLICENCE 700 1- Huang Fang School of Chemistry and Biochemistry, and Renewable Bioproducts Institute,, Georgia Institute of Technology,, 500 10th street N.W., 30332-0620, Atlanta, GA, USA aut NATIONALLICENCE 700 1- Kosa Matyas School of Chemistry and Biochemistry, and Renewable Bioproducts Institute,, Georgia Institute of Technology,, 500 10th street N.W., 30332-0620, Atlanta, GA, USA aut NATIONALLICENCE 700 1- Sun Qining School of Chemistry and Biochemistry, and Renewable Bioproducts Institute,, Georgia Institute of Technology,, 500 10th street N.W., 30332-0620, Atlanta, GA, USA aut NATIONALLICENCE 700 1- Meng Xianzhi School of Chemistry and Biochemistry, and Renewable Bioproducts Institute,, Georgia Institute of Technology,, 500 10th street N.W., 30332-0620, Atlanta, GA, USA aut NATIONALLICENCE 700 1- Huang Danlian College of Environmental Science and Engineering, Hunan University, 410082, Changsha, People's Republic of China aut NATIONALLICENCE 700 1- Ragauskas Arthur School of Chemistry and Biochemistry, and Renewable Bioproducts Institute,, Georgia Institute of Technology,, 500 10th street N.W., 30332-0620, Atlanta, GA, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/17(2015-09-01), 7369-7377 0175-7598 99:17<7369 2015 99 253