caa a22 4500 60550282X CHVBK 20210128100607.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00253-015-6664-4 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6664-4 A novel method to produce armored double-stranded DNA by encapsulation of MS2 viral capsids [Elektronische Daten] [Lei Zhang, Yu Sun, Le Chang, Tingting Jia, Guojing Wang, Rui Zhang, Kuo Zhang, Jinming Li] With the rapid development of molecular diagnostic techniques, there is a growing need for quality controls and standards with favorable properties to monitor the entire detection process. In this study, we describe a novel method to produce armored hepatitis B virus (HBV) and human papillomavirus (HPV) DNA for use in nucleic acid tests, which was confirmed to be stable, homogeneous, noninfectious, nuclease resistant, and safe for shipping. We demonstrated that MS2 bacteriophage could successfully package double-stranded DNA of 1.3-, 3-, 3.5-, and 6.5-kb length into viral capsids with high reassembly efficiency. This is the first application of RNA bacteriophage MS2 as a platform to encapsulate double-stranded DNA, forming virus-like particles (VLPs) which were indistinguishable from native MS2 capsids in size and morphology. Moreover, by analyzing the interaction mechanism of pac site and the MS2 coat protein (CP), we found that in addition to the recognized initiation signal TR-RNA, TR-DNA can also trigger spontaneous reassembly of CP dimers, providing a more convenient and feasible method of assembly. In conclusion, this straightforward and reliable manufacturing approach makes armored DNA an ideal control and standard for use in clinical laboratory tests and diagnostics, possessing prospects for broad application, especially providing a new platform for the production of quality controls for DNA viruses. Springer-Verlag Berlin Heidelberg, 2015 Armored double-stranded DNA nationallicence MS2 bacteriophage nationallicence Encapsulation nationallicence Virus-like particles nationallicence Quality controls nationallicence Zhang Lei Peking University Fifth School of Clinical Medicine, Beijing, People's Republic of China aut Sun Yu National Center for Clinical Laboratories, Beijing Hospital, Beijing, People's Republic of China aut Chang Le National Center for Clinical Laboratories, Beijing Hospital, Beijing, People's Republic of China aut Jia Tingting National Center for Clinical Laboratories, Beijing Hospital, Beijing, People's Republic of China aut Wang Guojing National Center for Clinical Laboratories, Beijing Hospital, Beijing, People's Republic of China aut Zhang Rui National Center for Clinical Laboratories, Beijing Hospital, Beijing, People's Republic of China aut Zhang Kuo National Center for Clinical Laboratories, Beijing Hospital, Beijing, People's Republic of China aut Li Jinming Peking University Fifth School of Clinical Medicine, Beijing, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/17(2015-09-01), 7047-7057 0175-7598 99:17<7047 2015 99 253 https://doi.org/10.1007/s00253-015-6664-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6664-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Lei Peking University Fifth School of Clinical Medicine, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Sun Yu National Center for Clinical Laboratories, Beijing Hospital, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Chang Le National Center for Clinical Laboratories, Beijing Hospital, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Jia Tingting National Center for Clinical Laboratories, Beijing Hospital, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Wang Guojing National Center for Clinical Laboratories, Beijing Hospital, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Rui National Center for Clinical Laboratories, Beijing Hospital, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Kuo National Center for Clinical Laboratories, Beijing Hospital, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Li Jinming Peking University Fifth School of Clinical Medicine, Beijing, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/17(2015-09-01), 7047-7057 0175-7598 99:17<7047 2015 99 253