Engineering Yarrowia lipolytica for production of medium-chain fatty acids
| LEADER | caa a22 4500 | ||
|---|---|---|---|
| 001 | 605502838 | ||
| 003 | CHVBK | ||
| 005 | 20210128100607.0 | ||
| 007 | cr unu---uuuuu | ||
| 008 | 210128e20150901xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-015-6764-1 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6764-1 | ||
| 245 | 0 | 0 | |a Engineering Yarrowia lipolytica for production of medium-chain fatty acids |h [Elektronische Daten] |c [Charles Rutter, Shuyan Zhang, Christopher Rao] |
| 520 | 3 | |a Lipids are naturally derived products that offer an attractive, renewable alternative to petroleum-based hydrocarbons. While naturally produced long-chain fatty acids can replace some petroleum analogs, medium-chain fatty acid would more closely match the desired physical and chemical properties of currently employed petroleum products. In this study, we engineered Yarrowia lipolytica, an oleaginous yeast that naturally produces lipids at high titers, to produce medium-chain fatty acids. Five different acyl-acyl carrier protein (ACP) thioesterases with specificity for medium-chain acyl-ACP molecules were expressed in Y. lipolytica, resulting in formation of either decanoic or octanoic acid. These novel fatty acid products were found to comprise up to 40% of the total cell lipids. Furthermore, the reduction in chain length resulted in a twofold increase in specific lipid productivity in these engineered strains. The medium-chain fatty acids were found to be incorporated into all lipid classes. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Oleaginous yeast |2 nationallicence | |
| 690 | 7 | |a Medium-chain fatty acids |2 nationallicence | |
| 690 | 7 | |a Acyl-ACP thioesterase |2 nationallicence | |
| 690 | 7 | |a Metabolic engineering |2 nationallicence | |
| 700 | 1 | |a Rutter |D Charles |u Department of Chemical and Biomolecular Engineering, University of Illinois-Urbana Champaign, 600 S. Mathews Ave, 61801, Urbana, IL, USA |4 aut | |
| 700 | 1 | |a Zhang |D Shuyan |u Department of Chemical and Biomolecular Engineering, University of Illinois-Urbana Champaign, 600 S. Mathews Ave, 61801, Urbana, IL, USA |4 aut | |
| 700 | 1 | |a Rao |D Christopher |u Department of Chemical and Biomolecular Engineering, University of Illinois-Urbana Champaign, 600 S. Mathews Ave, 61801, Urbana, IL, USA |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/17(2015-09-01), 7359-7368 |x 0175-7598 |q 99:17<7359 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6764-1 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6764-1 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Rutter |D Charles |u Department of Chemical and Biomolecular Engineering, University of Illinois-Urbana Champaign, 600 S. Mathews Ave, 61801, Urbana, IL, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhang |D Shuyan |u Department of Chemical and Biomolecular Engineering, University of Illinois-Urbana Champaign, 600 S. Mathews Ave, 61801, Urbana, IL, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Rao |D Christopher |u Department of Chemical and Biomolecular Engineering, University of Illinois-Urbana Champaign, 600 S. Mathews Ave, 61801, Urbana, IL, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/17(2015-09-01), 7359-7368 |x 0175-7598 |q 99:17<7359 |1 2015 |2 99 |o 253 | ||