caa a22 4500 605502838 CHVBK 20210128100607.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00253-015-6764-1 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6764-1 Engineering Yarrowia lipolytica for production of medium-chain fatty acids [Elektronische Daten] [Charles Rutter, Shuyan Zhang, Christopher Rao] Lipids are naturally derived products that offer an attractive, renewable alternative to petroleum-based hydrocarbons. While naturally produced long-chain fatty acids can replace some petroleum analogs, medium-chain fatty acid would more closely match the desired physical and chemical properties of currently employed petroleum products. In this study, we engineered Yarrowia lipolytica, an oleaginous yeast that naturally produces lipids at high titers, to produce medium-chain fatty acids. Five different acyl-acyl carrier protein (ACP) thioesterases with specificity for medium-chain acyl-ACP molecules were expressed in Y. lipolytica, resulting in formation of either decanoic or octanoic acid. These novel fatty acid products were found to comprise up to 40% of the total cell lipids. Furthermore, the reduction in chain length resulted in a twofold increase in specific lipid productivity in these engineered strains. The medium-chain fatty acids were found to be incorporated into all lipid classes. Springer-Verlag Berlin Heidelberg, 2015 Oleaginous yeast nationallicence Medium-chain fatty acids nationallicence Acyl-ACP thioesterase nationallicence Metabolic engineering nationallicence Rutter Charles Department of Chemical and Biomolecular Engineering, University of Illinois-Urbana Champaign, 600 S. Mathews Ave, 61801, Urbana, IL, USA aut Zhang Shuyan Department of Chemical and Biomolecular Engineering, University of Illinois-Urbana Champaign, 600 S. Mathews Ave, 61801, Urbana, IL, USA aut Rao Christopher Department of Chemical and Biomolecular Engineering, University of Illinois-Urbana Champaign, 600 S. Mathews Ave, 61801, Urbana, IL, USA aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/17(2015-09-01), 7359-7368 0175-7598 99:17<7359 2015 99 253 https://doi.org/10.1007/s00253-015-6764-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6764-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rutter Charles Department of Chemical and Biomolecular Engineering, University of Illinois-Urbana Champaign, 600 S. Mathews Ave, 61801, Urbana, IL, USA aut NATIONALLICENCE 700 1- Zhang Shuyan Department of Chemical and Biomolecular Engineering, University of Illinois-Urbana Champaign, 600 S. Mathews Ave, 61801, Urbana, IL, USA aut NATIONALLICENCE 700 1- Rao Christopher Department of Chemical and Biomolecular Engineering, University of Illinois-Urbana Champaign, 600 S. Mathews Ave, 61801, Urbana, IL, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/17(2015-09-01), 7359-7368 0175-7598 99:17<7359 2015 99 253