caa a22 4500 605502862 CHVBK 20210128100608.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00253-015-6775-y doi (NATIONALLICENCE)springer-10.1007/s00253-015-6775-y Visualization and analysis of EPS glycoconjugates of the thermoacidophilic archaeon Sulfolobus metallicus [Elektronische Daten] [Ruiyong Zhang, Thomas Neu, Yutong Zhang, Sören Bellenberg, Ute Kuhlicke, Qian Li, Wolfgang Sand, Mario Vera] Biofilms are surface-associated colonies of microorganisms embedded in a matrix of extracellular polymeric substances (EPS). As EPS mediate the contact between cells and surfaces, an understanding of their composition and production is of particular interest. In this study, the EPS components of Sulfolobus metallicus DSM 6482T forming biofilms on elemental sulfur (S0) were investigated by confocal laser scanning microscopy (CLSM). In order to visualize cell and EPS distributions, biofilm cells were stained with various dyes specific for glycoconjugates, proteins, nucleic acids and lipids. Biofilm cells on S0 were heterogeneously distributed and characterized as individual cells, microcolonies, and large clusters up to a hundred micrometers in diameter. The glycoconjugates in biofilms were detected by fluorescence lectin-binding analysis (FLBA). Screening of 72 commercially available lectins resulted in the selection of 21 lectins useful for staining biofilms of S. metallicus T. Capsular EPS from planktonic cells were mainly composed of carbohydrates and proteins. In contrast, colloidal EPS from planktonic cells were dominated by carbohydrates. Proteins were found to be major components in EPS from biofilms on S0. Using specific probes combined with CLSM, we showed that extracellular proteins and nucleic acids were present in the EPS matrix. Finally, we showed that S. metallicus T cells were embedded in a flexible EPS matrix. This study provides new insights into archaeal biofilms and EPS composition and properties with respect to their interactions with S0. Springer-Verlag Berlin Heidelberg, 2015 Biofilm nationallicence Glycoconjugates nationallicence Thermoacidophilic archaea nationallicence Lectin nationallicence Elemental sulfur nationallicence Zhang Ruiyong Aquatische Biotechnologie, Biofilm Centre, Universität Duisburg—Essen, Universitätsstraße 5, 45141, Essen, Germany aut Neu Thomas Department of River Ecology, Helmholtz Centre for Environmental Research—UFZ, Brückstraße 3a, 39114, Magdeburg, Germany aut Zhang Yutong Aquatische Biotechnologie, Biofilm Centre, Universität Duisburg—Essen, Universitätsstraße 5, 45141, Essen, Germany aut Bellenberg Sören Aquatische Biotechnologie, Biofilm Centre, Universität Duisburg—Essen, Universitätsstraße 5, 45141, Essen, Germany aut Kuhlicke Ute Department of River Ecology, Helmholtz Centre for Environmental Research—UFZ, Brückstraße 3a, 39114, Magdeburg, Germany aut Li Qian Aquatische Biotechnologie, Biofilm Centre, Universität Duisburg—Essen, Universitätsstraße 5, 45141, Essen, Germany aut Sand Wolfgang Aquatische Biotechnologie, Biofilm Centre, Universität Duisburg—Essen, Universitätsstraße 5, 45141, Essen, Germany aut Vera Mario Aquatische Biotechnologie, Biofilm Centre, Universität Duisburg—Essen, Universitätsstraße 5, 45141, Essen, Germany aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/17(2015-09-01), 7343-7356 0175-7598 99:17<7343 2015 99 253 https://doi.org/10.1007/s00253-015-6775-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6775-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Ruiyong Aquatische Biotechnologie, Biofilm Centre, Universität Duisburg—Essen, Universitätsstraße 5, 45141, Essen, Germany aut NATIONALLICENCE 700 1- Neu Thomas Department of River Ecology, Helmholtz Centre for Environmental Research—UFZ, Brückstraße 3a, 39114, Magdeburg, Germany aut NATIONALLICENCE 700 1- Zhang Yutong Aquatische Biotechnologie, Biofilm Centre, Universität Duisburg—Essen, Universitätsstraße 5, 45141, Essen, Germany aut NATIONALLICENCE 700 1- Bellenberg Sören Aquatische Biotechnologie, Biofilm Centre, Universität Duisburg—Essen, Universitätsstraße 5, 45141, Essen, Germany aut NATIONALLICENCE 700 1- Kuhlicke Ute Department of River Ecology, Helmholtz Centre for Environmental Research—UFZ, Brückstraße 3a, 39114, Magdeburg, Germany aut NATIONALLICENCE 700 1- Li Qian Aquatische Biotechnologie, Biofilm Centre, Universität Duisburg—Essen, Universitätsstraße 5, 45141, Essen, Germany aut NATIONALLICENCE 700 1- Sand Wolfgang Aquatische Biotechnologie, Biofilm Centre, Universität Duisburg—Essen, Universitätsstraße 5, 45141, Essen, Germany aut NATIONALLICENCE 700 1- Vera Mario Aquatische Biotechnologie, Biofilm Centre, Universität Duisburg—Essen, Universitätsstraße 5, 45141, Essen, Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/17(2015-09-01), 7343-7356 0175-7598 99:17<7343 2015 99 253