caa a22 4500 605502897 CHVBK 20210128100608.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00253-015-6468-6 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6468-6 Comparison of the biological activities of Saccharomyces cerevisiae -expressed intracellular EGF, extracellular EGF, and tagged EGF in early-weaned pigs [Elektronische Daten] [Shujin Wang, Chunhua Guo, Lin Zhou, Zhengfan Zhang, Yanling Huang, Jiabao Yang, Xue Bai, Kuanmin Yang] Epidermal growth factor (EGF) ameliorates stress and prevents incomplete gastrointestinal development in early-weaned piglets in commercial swine farming. This study aimed to further analyze the biological activities of intracellularly expressed EGF (IE-EGF), extracellularly expressed EGF (EE-EGF), and tagged EGF (T-EGF) from Saccharomyces cerevisiae in early-weaned pigs. In this study, we assigned 24 pigs to each of 5 groups that were provided a basic diet (the control group) or a diet supplemented with empty vector-expressing S. cerevisiae [the INVSc1(EV) group], T-EGF-expressing S. cerevisiae [the INVSc1-TE(−) group], EE-EGF-expressing S. cerevisiae [the INVSc1-EE(+) group], or IE-EGF-expressing S. cerevisiae [the INVSc1-IE(+) group]. All treatments were delivered at a dose of 60μg EGF/kg body weight (BW) everyday. All the piglets were sacrificed after 21day to determine their physio-biochemical indexes, immune functions, and intestinal development. In the piglet experiments, recombinant S. cerevisiae survived throughout the intestinal tract. The BW and intestinal development (e.g., mean villous height, crypt depth, villous height:crypt depth ratio (IVR), and total protein, DNA, and RNA contents) of the piglets were significantly enhanced in the INVSc1-IE(+) group compared with the animals in the INVSc1-EE(+) and INVSc1-TE(−) groups (P < 0.05). In addition, increased proliferating cell nuclear antigen (PCNA) staining was observed in the piglets that received the INVSc1-IE(+) treatment (approximately 80%) compared with those that received the INVSc1-TE(−) (approximately 70%) and INVSc1-EE(+) treatments (approximately 70%). The levels of lactate dehydrogenase (LDH), creatine kinase (CK), alkaline phosphatase (ALP), immunoglobulin A (IgA), immunoglobulin M (IgM), and immunoglobulin G (IgG) were also significantly increased in the INVSc1-IE(+) group compared with the INVSc1-EE(+) and INVSc1-TE(−) groups (P < 0.05). Furthermore, the proliferation of piglet enterocytes was also significantly stimulated by both IE-EGF and EE-EGF compared with T-EGF in vitro (P < 0.05). Our data further demonstrate the previously reported hypothesis that IE-EGF is more suitable than EE-EGF or T-EGF for applications in early-weaned pigs. Springer-Verlag Berlin Heidelberg, 2015 Biological activity nationallicence Early-weaned pig nationallicence Epidermal growth factor nationallicence Saccharomyces cerevisiae nationallicence Application nationallicence Wang Shujin College of Life Science and Technology, Southwest University for Nationalities, 610041, Chengdu, People's Republic of China aut Guo Chunhua College of Life Science and Technology, Southwest University for Nationalities, 610041, Chengdu, People's Republic of China aut Zhou Lin Shenzhen Premixinve Nutrition Co., LTD, 518103, Shenzhen, People's Republic of China aut Zhang Zhengfan College of Life Science and Technology, Southwest University for Nationalities, 610041, Chengdu, People's Republic of China aut Huang Yanling College of Life Science and Technology, Southwest University for Nationalities, 610041, Chengdu, People's Republic of China aut Yang Jiabao Animal Science Research Institute of Sichuan Province, 610041, Chengdu, People's Republic of China aut Bai Xue College of Life Science and Technology, Southwest University for Nationalities, 610041, Chengdu, People's Republic of China aut Yang Kuanmin Shenzhen Premixinve Nutrition Co., LTD, 518103, Shenzhen, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/17(2015-09-01), 7125-7135 0175-7598 99:17<7125 2015 99 253 https://doi.org/10.1007/s00253-015-6468-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6468-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Shujin College of Life Science and Technology, Southwest University for Nationalities, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Guo Chunhua College of Life Science and Technology, Southwest University for Nationalities, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Zhou Lin Shenzhen Premixinve Nutrition Co., LTD, 518103, Shenzhen, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Zhengfan College of Life Science and Technology, Southwest University for Nationalities, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Huang Yanling College of Life Science and Technology, Southwest University for Nationalities, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Yang Jiabao Animal Science Research Institute of Sichuan Province, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Bai Xue College of Life Science and Technology, Southwest University for Nationalities, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Yang Kuanmin Shenzhen Premixinve Nutrition Co., LTD, 518103, Shenzhen, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/17(2015-09-01), 7125-7135 0175-7598 99:17<7125 2015 99 253