caa a22 4500 605502900 CHVBK 20210128100608.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00253-015-6784-x doi (NATIONALLICENCE)springer-10.1007/s00253-015-6784-x Assessing hog lagoon waste contamination in the Cape Fear Watershed using Bacteroidetes 16S rRNA gene pyrosequencing [Elektronische Daten] [Ann Arfken, Bongkeun Song, Michael Mallin] Hog lagoons can be major sources of waste and nutrient contamination to watersheds adjacent to pig farms. Fecal source tracking methods targeting Bacteroidetes 16S rRNA genes in pig fecal matter may underestimate or fail to detect hog lagoon contamination in riverine environments. In order to detect hog lagoon wastewater contamination in the Cape Fear Watershed, where a large number of hog farms are present, we conducted pyrosequencing analyses of Bacteroidetes 16S rRNA genes in hog lagoon waste and identified new hog lagoon-specific marker sequences. Additional pyrosequencing analyses of Bacteroidetes 16S rRNA genes were conducted with surface water samples collected at 4 sites during 5months in the Cape Fear Watershed. Using an operational taxonomic unit (OTU) identity cutoff value of 97%, these newly identified hog lagoon markers were found in 3 of the river samples, while only 1 sample contained the pig fecal marker. In the sample containing the pig fecal marker, there was a relatively high percentage (14.1%) of the hog lagoon markers and a low pig fecal marker relative abundance of 0.4%in the Bacteroidetes 16S rRNA gene sequences. This suggests that hog lagoon contamination must be somewhat significant in order for pig fecal markers to be detected, and low levels of hog lagoon contamination cannot be detected targeting only pig-specific fecal markers. Thus, new hog lagoon markers havea better detection capacity for lagoon waste contamination, and in conjunction with a pig fecal marker, provide a more comprehensive and accurate detection of hog lagoon waste contamination in susceptible watersheds. Springer-Verlag Berlin Heidelberg, 2015 Microbial source tracking nationallicence Bacteroidetes nationallicence Fecal Pollution nationallicence Arfken Ann University of North Carolina Wilmington, 601 S. College Rd, Wilmington, NC, USA aut Song Bongkeun University of North Carolina Wilmington, 601 S. College Rd, Wilmington, NC, USA aut Mallin Michael University of North Carolina Wilmington, 601 S. College Rd, Wilmington, NC, USA aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/17(2015-09-01), 7283-7293 0175-7598 99:17<7283 2015 99 253 https://doi.org/10.1007/s00253-015-6784-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6784-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Arfken Ann University of North Carolina Wilmington, 601 S. College Rd, Wilmington, NC, USA aut NATIONALLICENCE 700 1- Song Bongkeun University of North Carolina Wilmington, 601 S. College Rd, Wilmington, NC, USA aut NATIONALLICENCE 700 1- Mallin Michael University of North Carolina Wilmington, 601 S. College Rd, Wilmington, NC, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/17(2015-09-01), 7283-7293 0175-7598 99:17<7283 2015 99 253