Endogenic oxidative stress response contributes to glutathione over-accumulation in mutant Saccharomyces cerevisiae Y518
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6629-7 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6629-7 | ||
| 245 | 0 | 0 | |a Endogenic oxidative stress response contributes to glutathione over-accumulation in mutant Saccharomyces cerevisiae Y518 |h [Elektronische Daten] |c [Yibo Zhu, Jiang Sun, Yingyue Zhu, Limei Wang, Bin Qi] |
| 520 | 3 | |a Mechanisms of glutathione (GSH) over-accumulation in mutant Saccharomyces cerevisiae Y518 screened by ultraviolet and nitrosoguanidine-induced random mutagenesis were studied. Y518 accumulated higher levels of GSH and l-cysteine than its wild-type strain. RNA-Seq and pathway enrichment analysis indicated a difference in the expression of key genes involved in cysteine production, the GSH biosynthesis pathway, and antioxidation processes. GSH1, MET17, CYS4, GPX2, CTT1, TRX2, and SOD1 and the transcriptional activators SKN7 and YAP1 were up-regulated in the mutant. Moreover, Y518 showed a dysfunctional respiratory chain resulting from dramatically weakened activity of complex III and significant elevation of intracellular reactive oxygen species (ROS) levels. The supplementation of antimycin A in the culture of the parent strain showed equivalent changes of ROS and GSH level. This study indicates that defective complex III prompts abundant endogenic ROS generation, which triggers an oxidative stress response and upregulation of gene expression associated with GSH biosynthesis. This finding may be helpful for developing new strategies for GSH fermentation process optimization or metabolic engineering. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Glutathione |2 nationallicence | |
| 690 | 7 | |a Endogenic reactive oxygen stress |2 nationallicence | |
| 690 | 7 | |a RNA-Seq |2 nationallicence | |
| 690 | 7 | |a Malfunctional respiratory chain |2 nationallicence | |
| 690 | 7 | |a Saccharomyces cerevisiae |2 nationallicence | |
| 700 | 1 | |a Zhu |D Yibo |u School of Biotechnology and Food Engineering, Changshu Institute of Technology, 215500, Changshu, Jiangsu, China |4 aut | |
| 700 | 1 | |a Sun |D Jiang |u School of Biotechnology and Food Engineering, Changshu Institute of Technology, 215500, Changshu, Jiangsu, China |4 aut | |
| 700 | 1 | |a Zhu |D Yingyue |u School of Biotechnology and Food Engineering, Changshu Institute of Technology, 215500, Changshu, Jiangsu, China |4 aut | |
| 700 | 1 | |a Wang |D Limei |u School of Biotechnology and Food Engineering, Changshu Institute of Technology, 215500, Changshu, Jiangsu, China |4 aut | |
| 700 | 1 | |a Qi |D Bin |u School of Biotechnology and Food Engineering, Changshu Institute of Technology, 215500, Changshu, Jiangsu, China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/17(2015-09-01), 7069-7078 |x 0175-7598 |q 99:17<7069 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6629-7 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6629-7 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhu |D Yibo |u School of Biotechnology and Food Engineering, Changshu Institute of Technology, 215500, Changshu, Jiangsu, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sun |D Jiang |u School of Biotechnology and Food Engineering, Changshu Institute of Technology, 215500, Changshu, Jiangsu, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhu |D Yingyue |u School of Biotechnology and Food Engineering, Changshu Institute of Technology, 215500, Changshu, Jiangsu, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wang |D Limei |u School of Biotechnology and Food Engineering, Changshu Institute of Technology, 215500, Changshu, Jiangsu, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Qi |D Bin |u School of Biotechnology and Food Engineering, Changshu Institute of Technology, 215500, Changshu, Jiangsu, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/17(2015-09-01), 7069-7078 |x 0175-7598 |q 99:17<7069 |1 2015 |2 99 |o 253 | ||