Effects of β-cyclodextrin and methyl jasmonate on the production of vindoline, catharanthine, and ajmalicine in Catharanthus roseus cambial meristematic cell cultures
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6651-9 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6651-9 | ||
| 245 | 0 | 0 | |a Effects of β-cyclodextrin and methyl jasmonate on the production of vindoline, catharanthine, and ajmalicine in Catharanthus roseus cambial meristematic cell cultures |h [Elektronische Daten] |c [Pengfei Zhou, Jiazeng Yang, Jianhua Zhu, Shuijie He, Wenjin Zhang, Rongmin Yu, Jiachen Zi, Liyan Song, Xuesong Huang] |
| 520 | 3 | |a Long-term stable cell growth and production of vindoline, catharanthine, and ajmalicine of cambial meristematic cells (CMCs) from Catharanthus roseus were observed after 2years of culture. C. roseus CMCs were treated with β-cyclodextrin (β-CD) and methyl jasmonate (MeJA) individually or in combination and were cultured both in conventional Erlenmeyer flasks (100, 250, and 500mL) and in a 5-L stirred hybrid airlift bioreactor. CMCs of C. roseus cultured in the bioreactor showed higher yields of vindoline, catharanthine, and ajmalicine than those cultured in flasks. CMCs of C. roseus cultured in the bioreactor and treated with 10mM β-CD and 150μM MeJA gave the highest yields of vindoline (7.45mg/L), catharanthine (1.76mg/L), and ajmalicine (58.98mg/L), concentrations that were 799, 654, and 426% higher, respectively, than yields of CMCs cultured in 100-mL flasks without elicitors. Quantitative reverse transcription (RT)-PCR showed that β-CD and MeJA upregulated transcription levels of genes related to the biosynthesis of terpenoid indole alkaloids (TIAs). This is the first study to report that β-CD induced the generation of NO, which plays an important role in mediating the production of TIAs in C. roseus CMCs. These results suggest that β-CD and MeJA can enhance the production of TIAs in CMCs of C. roseus, and thus, CMCs of C. roseus have significant potential to be an industrial platform for production of bioactive alkaloids. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Catharanthus roseus |2 nationallicence | |
| 690 | 7 | |a Cambial meristematic cells |2 nationallicence | |
| 690 | 7 | |a β-Cyclodextrin |2 nationallicence | |
| 690 | 7 | |a Methyl jasmonate |2 nationallicence | |
| 690 | 7 | |a Terpenoid indole alkaloids |2 nationallicence | |
| 700 | 1 | |a Zhou |D Pengfei |u Biotechnological Institute of Chinese Materia Medica, Jinan University, 510632, Guangzhou, China |4 aut | |
| 700 | 1 | |a Yang |D Jiazeng |u Biotechnological Institute of Chinese Materia Medica, Jinan University, 510632, Guangzhou, China |4 aut | |
| 700 | 1 | |a Zhu |D Jianhua |u Department of Natural Products Chemistry, College of Pharmacy, Jinan University, 510632, Guangzhou, China |4 aut | |
| 700 | 1 | |a He |D Shuijie |u Biotechnological Institute of Chinese Materia Medica, Jinan University, 510632, Guangzhou, China |4 aut | |
| 700 | 1 | |a Zhang |D Wenjin |u Biotechnological Institute of Chinese Materia Medica, Jinan University, 510632, Guangzhou, China |4 aut | |
| 700 | 1 | |a Yu |D Rongmin |u Biotechnological Institute of Chinese Materia Medica, Jinan University, 510632, Guangzhou, China |4 aut | |
| 700 | 1 | |a Zi |D Jiachen |u Biotechnological Institute of Chinese Materia Medica, Jinan University, 510632, Guangzhou, China |4 aut | |
| 700 | 1 | |a Song |D Liyan |u Biotechnological Institute of Chinese Materia Medica, Jinan University, 510632, Guangzhou, China |4 aut | |
| 700 | 1 | |a Huang |D Xuesong |u Department of Food Science and Engineering, College of Science and Engineering, Jinan University, 510632, Guangzhou, China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/17(2015-09-01), 7035-7045 |x 0175-7598 |q 99:17<7035 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6651-9 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6651-9 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhou |D Pengfei |u Biotechnological Institute of Chinese Materia Medica, Jinan University, 510632, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yang |D Jiazeng |u Biotechnological Institute of Chinese Materia Medica, Jinan University, 510632, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhu |D Jianhua |u Department of Natural Products Chemistry, College of Pharmacy, Jinan University, 510632, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a He |D Shuijie |u Biotechnological Institute of Chinese Materia Medica, Jinan University, 510632, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhang |D Wenjin |u Biotechnological Institute of Chinese Materia Medica, Jinan University, 510632, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yu |D Rongmin |u Biotechnological Institute of Chinese Materia Medica, Jinan University, 510632, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zi |D Jiachen |u Biotechnological Institute of Chinese Materia Medica, Jinan University, 510632, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Song |D Liyan |u Biotechnological Institute of Chinese Materia Medica, Jinan University, 510632, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Huang |D Xuesong |u Department of Food Science and Engineering, College of Science and Engineering, Jinan University, 510632, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/17(2015-09-01), 7035-7045 |x 0175-7598 |q 99:17<7035 |1 2015 |2 99 |o 253 | ||