caa a22 4500 605502951 CHVBK 20210128100608.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00253-015-6651-9 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6651-9 Effects of β-cyclodextrin and methyl jasmonate on the production of vindoline, catharanthine, and ajmalicine in Catharanthus roseus cambial meristematic cell cultures [Elektronische Daten] [Pengfei Zhou, Jiazeng Yang, Jianhua Zhu, Shuijie He, Wenjin Zhang, Rongmin Yu, Jiachen Zi, Liyan Song, Xuesong Huang] Long-term stable cell growth and production of vindoline, catharanthine, and ajmalicine of cambial meristematic cells (CMCs) from Catharanthus roseus were observed after 2years of culture. C. roseus CMCs were treated with β-cyclodextrin (β-CD) and methyl jasmonate (MeJA) individually or in combination and were cultured both in conventional Erlenmeyer flasks (100, 250, and 500mL) and in a 5-L stirred hybrid airlift bioreactor. CMCs of C. roseus cultured in the bioreactor showed higher yields of vindoline, catharanthine, and ajmalicine than those cultured in flasks. CMCs of C. roseus cultured in the bioreactor and treated with 10mM β-CD and 150μM MeJA gave the highest yields of vindoline (7.45mg/L), catharanthine (1.76mg/L), and ajmalicine (58.98mg/L), concentrations that were 799, 654, and 426% higher, respectively, than yields of CMCs cultured in 100-mL flasks without elicitors. Quantitative reverse transcription (RT)-PCR showed that β-CD and MeJA upregulated transcription levels of genes related to the biosynthesis of terpenoid indole alkaloids (TIAs). This is the first study to report that β-CD induced the generation of NO, which plays an important role in mediating the production of TIAs in C. roseus CMCs. These results suggest that β-CD and MeJA can enhance the production of TIAs in CMCs of C. roseus, and thus, CMCs of C. roseus have significant potential to be an industrial platform for production of bioactive alkaloids. Springer-Verlag Berlin Heidelberg, 2015 Catharanthus roseus nationallicence Cambial meristematic cells nationallicence β-Cyclodextrin nationallicence Methyl jasmonate nationallicence Terpenoid indole alkaloids nationallicence Zhou Pengfei Biotechnological Institute of Chinese Materia Medica, Jinan University, 510632, Guangzhou, China aut Yang Jiazeng Biotechnological Institute of Chinese Materia Medica, Jinan University, 510632, Guangzhou, China aut Zhu Jianhua Department of Natural Products Chemistry, College of Pharmacy, Jinan University, 510632, Guangzhou, China aut He Shuijie Biotechnological Institute of Chinese Materia Medica, Jinan University, 510632, Guangzhou, China aut Zhang Wenjin Biotechnological Institute of Chinese Materia Medica, Jinan University, 510632, Guangzhou, China aut Yu Rongmin Biotechnological Institute of Chinese Materia Medica, Jinan University, 510632, Guangzhou, China aut Zi Jiachen Biotechnological Institute of Chinese Materia Medica, Jinan University, 510632, Guangzhou, China aut Song Liyan Biotechnological Institute of Chinese Materia Medica, Jinan University, 510632, Guangzhou, China aut Huang Xuesong Department of Food Science and Engineering, College of Science and Engineering, Jinan University, 510632, Guangzhou, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/17(2015-09-01), 7035-7045 0175-7598 99:17<7035 2015 99 253 https://doi.org/10.1007/s00253-015-6651-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6651-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhou Pengfei Biotechnological Institute of Chinese Materia Medica, Jinan University, 510632, Guangzhou, China aut NATIONALLICENCE 700 1- Yang Jiazeng Biotechnological Institute of Chinese Materia Medica, Jinan University, 510632, Guangzhou, China aut NATIONALLICENCE 700 1- Zhu Jianhua Department of Natural Products Chemistry, College of Pharmacy, Jinan University, 510632, Guangzhou, China aut NATIONALLICENCE 700 1- He Shuijie Biotechnological Institute of Chinese Materia Medica, Jinan University, 510632, Guangzhou, China aut NATIONALLICENCE 700 1- Zhang Wenjin Biotechnological Institute of Chinese Materia Medica, Jinan University, 510632, Guangzhou, China aut NATIONALLICENCE 700 1- Yu Rongmin Biotechnological Institute of Chinese Materia Medica, Jinan University, 510632, Guangzhou, China aut NATIONALLICENCE 700 1- Zi Jiachen Biotechnological Institute of Chinese Materia Medica, Jinan University, 510632, Guangzhou, China aut NATIONALLICENCE 700 1- Song Liyan Biotechnological Institute of Chinese Materia Medica, Jinan University, 510632, Guangzhou, China aut NATIONALLICENCE 700 1- Huang Xuesong Department of Food Science and Engineering, College of Science and Engineering, Jinan University, 510632, Guangzhou, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/17(2015-09-01), 7035-7045 0175-7598 99:17<7035 2015 99 253