A novel bio-functional material based on mammalian cell aggresomes
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6684-0 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6684-0 | ||
| 245 | 0 | 2 | |a A novel bio-functional material based on mammalian cell aggresomes |h [Elektronische Daten] |c [Escarlata Rodríguez-Carmona, Rosa Mendoza, Eugènia Ruiz-Cánovas, Neus Ferrer-Miralles, Ibane Abasolo, Simó Schwartz Jr, Antonio Villaverde, José Corchero] |
| 520 | 3 | |a Aggresomes are protein aggregates found in mammalian cells when the intracellular protein degradation machinery is over-titered. Despite that they abound in cells producing recombinant proteins of biomedical and biotechnological interest, the physiological roles of these protein clusters and the functional status of the embedded proteins remain basically unexplored. In this work, we have determined for the first time that, like in bacterial inclusion bodies, deposition of recombinant proteins into aggresomes does not imply functional inactivation. As a model, human α-galactosidase A (GLA) has been expressed in mammalian cells as enzymatically active, mechanically stable aggresomes showing higher thermal stability than the soluble GLA version. Since aggresomes are easily produced and purified, we propose these particles as novel functional biomaterials with potential as carrier-free, self-immobilized catalyzers in biotechnology and biomedicine. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Recombinant protein |2 nationallicence | |
| 690 | 7 | |a Protein nanoparticles |2 nationallicence | |
| 690 | 7 | |a Immobilized catalyzer |2 nationallicence | |
| 690 | 7 | |a Aggresomes |2 nationallicence | |
| 700 | 1 | |a Rodríguez-Carmona |D Escarlata |u Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, 08193, Barcelona, Spain |4 aut | |
| 700 | 1 | |a Mendoza |D Rosa |u CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona, Spain |4 aut | |
| 700 | 1 | |a Ruiz-Cánovas |D Eugènia |u Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, Bellaterra, 08193, Barcelona, Spain |4 aut | |
| 700 | 1 | |a Ferrer-Miralles |D Neus |u Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, 08193, Barcelona, Spain |4 aut | |
| 700 | 1 | |a Abasolo |D Ibane |u CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona, Spain |4 aut | |
| 700 | 1 | |a Schwartz Jr |D Simó |u CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona, Spain |4 aut | |
| 700 | 1 | |a Villaverde |D Antonio |u Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, 08193, Barcelona, Spain |4 aut | |
| 700 | 1 | |a Corchero |D José |u Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, 08193, Barcelona, Spain |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/17(2015-09-01), 7079-7088 |x 0175-7598 |q 99:17<7079 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6684-0 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6684-0 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Rodríguez-Carmona |D Escarlata |u Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, 08193, Barcelona, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Mendoza |D Rosa |u CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ruiz-Cánovas |D Eugènia |u Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, Bellaterra, 08193, Barcelona, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ferrer-Miralles |D Neus |u Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, 08193, Barcelona, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Abasolo |D Ibane |u CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Schwartz Jr |D Simó |u CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Villaverde |D Antonio |u Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, 08193, Barcelona, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Corchero |D José |u Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, 08193, Barcelona, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/17(2015-09-01), 7079-7088 |x 0175-7598 |q 99:17<7079 |1 2015 |2 99 |o 253 | ||