caa a22 4500 605502978 CHVBK 20210128100608.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00253-015-6684-0 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6684-0 A novel bio-functional material based on mammalian cell aggresomes [Elektronische Daten] [Escarlata Rodríguez-Carmona, Rosa Mendoza, Eugènia Ruiz-Cánovas, Neus Ferrer-Miralles, Ibane Abasolo, Simó Schwartz Jr, Antonio Villaverde, José Corchero] Aggresomes are protein aggregates found in mammalian cells when the intracellular protein degradation machinery is over-titered. Despite that they abound in cells producing recombinant proteins of biomedical and biotechnological interest, the physiological roles of these protein clusters and the functional status of the embedded proteins remain basically unexplored. In this work, we have determined for the first time that, like in bacterial inclusion bodies, deposition of recombinant proteins into aggresomes does not imply functional inactivation. As a model, human α-galactosidase A (GLA) has been expressed in mammalian cells as enzymatically active, mechanically stable aggresomes showing higher thermal stability than the soluble GLA version. Since aggresomes are easily produced and purified, we propose these particles as novel functional biomaterials with potential as carrier-free, self-immobilized catalyzers in biotechnology and biomedicine. Springer-Verlag Berlin Heidelberg, 2015 Recombinant protein nationallicence Protein nanoparticles nationallicence Immobilized catalyzer nationallicence Aggresomes nationallicence Rodríguez-Carmona Escarlata Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, 08193, Barcelona, Spain aut Mendoza Rosa CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona, Spain aut Ruiz-Cánovas Eugènia Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, Bellaterra, 08193, Barcelona, Spain aut Ferrer-Miralles Neus Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, 08193, Barcelona, Spain aut Abasolo Ibane CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona, Spain aut Schwartz Jr Simó CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona, Spain aut Villaverde Antonio Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, 08193, Barcelona, Spain aut Corchero José Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, 08193, Barcelona, Spain aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/17(2015-09-01), 7079-7088 0175-7598 99:17<7079 2015 99 253 https://doi.org/10.1007/s00253-015-6684-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6684-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rodríguez-Carmona Escarlata Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, 08193, Barcelona, Spain aut NATIONALLICENCE 700 1- Mendoza Rosa CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona, Spain aut NATIONALLICENCE 700 1- Ruiz-Cánovas Eugènia Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, Bellaterra, 08193, Barcelona, Spain aut NATIONALLICENCE 700 1- Ferrer-Miralles Neus Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, 08193, Barcelona, Spain aut NATIONALLICENCE 700 1- Abasolo Ibane CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona, Spain aut NATIONALLICENCE 700 1- Schwartz Jr Simó CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona, Spain aut NATIONALLICENCE 700 1- Villaverde Antonio Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, 08193, Barcelona, Spain aut NATIONALLICENCE 700 1- Corchero José Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, 08193, Barcelona, Spain aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/17(2015-09-01), 7079-7088 0175-7598 99:17<7079 2015 99 253