caa a22 4500 60550301X CHVBK 20210128100608.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00253-015-6604-3 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6604-3 A plasmid-born Rap-Phr system regulates surfactin production, sporulation and genetic competence in the heterologous host, Bacillus subtilis OKB105 [Elektronische Daten] [Yang Yang, Hui-Jun Wu, Ling Lin, Qing-qing Zhu, Rainer Borriss, Xue-Wen Gao] According to the change of environment, soil-dwelling Bacillus species differentiate into distinct subpopulations, such as spores and competent cells. Rap-Phr systems have been found to be involved in this differentiation circuit by interacting with major regulatory proteins, such as Spo0A, ComA, and DegU. In this study, we report that the plasmid-born RapQ-PhrQ system found in Bacillus amyloliquefaciens B3 affects three regulatory pathways in the heterologous host Bacillus subtilis. Expression of rapQ in B. subtilis OKB105 strongly suppressed its sporulation efficiency, transformation efficiency, and surfactin production. Co-expression of phrQ or addition of synthesized PhrQ pentapeptide in vitro could compensate for the suppressive effects caused by rapQ. We also found that expression of rapQ decreased the transcriptional level of the sporulation-related gene spoIIE and surfactin synthesis-related gene srfA; meanwhile, the transcriptional levels of these genes could be rescued by co-expression of phrQ and in vitro addition of PhrQ pentapeptide. Electrophoretic mobility shift (EMSA) result also showed that RapQ could bind to ComA without interacting with ComA binding to DNA, and PhrQ pentapeptide antagonized RapQ activity in vitro. These results indicate that this new plasmid-born RapQ-PhrQ system controls sporulation, competent cell formation, and surfactin production in B. subtilis OKB105. Springer-Verlag Berlin Heidelberg, 2015 Rap-Phr system nationallicence Bacillus subtilis nationallicence Sporulation nationallicence Surfactin nationallicence Genetic competence nationallicence Yang Yang Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China aut Wu Hui-Jun Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China aut Lin Ling Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China aut Zhu Qing-qing Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China aut Borriss Rainer ABiTEP GmbH, Berlin, Germany aut Gao Xue-Wen Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/17(2015-09-01), 7241-7252 0175-7598 99:17<7241 2015 99 253 https://doi.org/10.1007/s00253-015-6604-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6604-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Yang Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Wu Hui-Jun Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Lin Ling Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Zhu Qing-qing Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Borriss Rainer ABiTEP GmbH, Berlin, Germany aut NATIONALLICENCE 700 1- Gao Xue-Wen Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/17(2015-09-01), 7241-7252 0175-7598 99:17<7241 2015 99 253