A GH57 4-α-glucanotransferase of hyperthermophilic origin with potential for alkyl glycoside production
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6435-2 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6435-2 | ||
| 245 | 0 | 2 | |a A GH57 4-α-glucanotransferase of hyperthermophilic origin with potential for alkyl glycoside production |h [Elektronische Daten] |c [Catherine Paul, Hans Leemhuis, Justyna Dobruchowska, Carl Grey, Linda Önnby, Sander van Leeuwen, Lubbert Dijkhuizen, Eva Karlsson] |
| 520 | 3 | |a 4-α-Glucanotransferase (GTase) enzymes (EC 2.4.1.25) modulate the size of α-glucans by cleaving and reforming α-1,4 glycosidic bonds in α-glucans, an essential process in starch and glycogen metabolism in plants and microorganisms. The glycoside hydrolase family 57 enzyme (GTase57) studied in the current work catalyzes both disproportionation and cyclization reactions. Amylose was converted into cyclic amylose (with a minimum size of 17 glucose monomers) as well as to a spectrum of maltodextrins, but in contrast to glycoside hydrolase family 13 cyclodextrin glucanotransferases (CGTases), no production of cyclodextrins (C6-C8) was observed. GTase57 also effectively produced alkyl-glycosides with long α-glucan chains from dodecyl-β-D-maltoside and starch, demonstrating the potential of the enzyme to produce novel variants of surfactants. Importantly, the GTase57 has excellent thermostability with a maximal activity at 95°C and an activity half-life of 150min at 90°C which is highly advantageous in this manufacturing process suggesting that enzymes from this relatively uncharacterized family, GH57, can be powerful biocatalysts for the production of large head group glucosides from soluble starch. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Amylomaltase |2 nationallicence | |
| 690 | 7 | |a Amylose |2 nationallicence | |
| 690 | 7 | |a Glucan |2 nationallicence | |
| 690 | 7 | |a Glycoside hydrolase |2 nationallicence | |
| 690 | 7 | |a Starch |2 nationallicence | |
| 690 | 7 | |a Alkyl glycoside |2 nationallicence | |
| 690 | 7 | |a MalQ |2 nationallicence | |
| 700 | 1 | |a Paul |D Catherine |u Biotechnology, Department of Chemistry, Lund University, Getingevägen 60, 22241, Lund, Sweden |4 aut | |
| 700 | 1 | |a Leemhuis |D Hans |u Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), Carbohydrate Competence Center (CCC), University of Groningen, Groningen, Netherlands |4 aut | |
| 700 | 1 | |a Dobruchowska |D Justyna |u Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), Carbohydrate Competence Center (CCC), University of Groningen, Groningen, Netherlands |4 aut | |
| 700 | 1 | |a Grey |D Carl |u Biotechnology, Department of Chemistry, Lund University, Getingevägen 60, 22241, Lund, Sweden |4 aut | |
| 700 | 1 | |a Önnby |D Linda |u Biotechnology, Department of Chemistry, Lund University, Getingevägen 60, 22241, Lund, Sweden |4 aut | |
| 700 | 1 | |a van Leeuwen |D Sander |u Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), Carbohydrate Competence Center (CCC), University of Groningen, Groningen, Netherlands |4 aut | |
| 700 | 1 | |a Dijkhuizen |D Lubbert |u Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), Carbohydrate Competence Center (CCC), University of Groningen, Groningen, Netherlands |4 aut | |
| 700 | 1 | |a Karlsson |D Eva |u Biotechnology, Department of Chemistry, Lund University, Getingevägen 60, 22241, Lund, Sweden |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/17(2015-09-01), 7101-7113 |x 0175-7598 |q 99:17<7101 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6435-2 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6435-2 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Paul |D Catherine |u Biotechnology, Department of Chemistry, Lund University, Getingevägen 60, 22241, Lund, Sweden |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Leemhuis |D Hans |u Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), Carbohydrate Competence Center (CCC), University of Groningen, Groningen, Netherlands |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Dobruchowska |D Justyna |u Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), Carbohydrate Competence Center (CCC), University of Groningen, Groningen, Netherlands |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Grey |D Carl |u Biotechnology, Department of Chemistry, Lund University, Getingevägen 60, 22241, Lund, Sweden |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Önnby |D Linda |u Biotechnology, Department of Chemistry, Lund University, Getingevägen 60, 22241, Lund, Sweden |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a van Leeuwen |D Sander |u Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), Carbohydrate Competence Center (CCC), University of Groningen, Groningen, Netherlands |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Dijkhuizen |D Lubbert |u Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), Carbohydrate Competence Center (CCC), University of Groningen, Groningen, Netherlands |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Karlsson |D Eva |u Biotechnology, Department of Chemistry, Lund University, Getingevägen 60, 22241, Lund, Sweden |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/17(2015-09-01), 7101-7113 |x 0175-7598 |q 99:17<7101 |1 2015 |2 99 |o 253 | ||