caa a22 4500 605503060 CHVBK 20210128100609.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00253-015-6455-y doi (NATIONALLICENCE)springer-10.1007/s00253-015-6455-y Improvement of atopic dermatitis-like skin lesions by IL-4 inhibition of P14 protein isolated from Lactobacillus casei in NC/Nga mice [Elektronische Daten] [Min-Soo Kim, Jin-Eung Kim, Yeo-Sang Yoon, Tai Kim, Jae-Gu Seo, Myung-Jun Chung, Do-Young Yum] Atopic dermatitis (AD) is a chronic inflammatory skin disease, with a complex etiology encompassing immunologic responses. AD is frequently associated with elevated serum immunoglobulin (Ig) E levels and is exacerbated by a variety of environmental factors, which contribute to its pathogenesis. However, the etiology of AD remains unknown. Recently, reports have documented the role of lactic acid bacteria (LAB) in the treatment and prevention of AD in humans and mice. The LAB, Lactobacillus casei (LC), is frequently used in the treatment of AD. To identify the active component of LC, we screened fractions obtained from the ion exchange chromatography of LC extracts. Using this approach, we identified the candidate protein, P14. We examined whether the P14 protein has anti-atopic properties, using both in vitro and in vivo models. Our results showed that the P14 protein selectively downregulated serum IgE and interleukin-4 cytokine levels, as well as the AD index and scratching score in AD-like NC/Nga mice. In addition, histological examination was also effective in mice. These results suggest that the P14 protein has potential therapeutic effects and that it may also serve as an effective immunomodulatory agent for treating patients with AD. Springer-Verlag Berlin Heidelberg, 2015 Atopic dermatitis nationallicence P14 protein nationallicence Immunoglobulin E nationallicence Interleukin-4 nationallicence NC/Nga mouse nationallicence Kim Min-Soo R&D Center, Cell Biotech, Co., Ltd, 134 Gaegok-Ri Wolgot-Myeon, 415-872, Gimpo-Si, Gyeonggi-Do, Korea aut Kim Jin-Eung R&D Center, Cell Biotech, Co., Ltd, 134 Gaegok-Ri Wolgot-Myeon, 415-872, Gimpo-Si, Gyeonggi-Do, Korea aut Yoon Yeo-Sang R&D Center, Cell Biotech, Co., Ltd, 134 Gaegok-Ri Wolgot-Myeon, 415-872, Gimpo-Si, Gyeonggi-Do, Korea aut Kim Tai R&D Center, Cell Biotech, Co., Ltd, 134 Gaegok-Ri Wolgot-Myeon, 415-872, Gimpo-Si, Gyeonggi-Do, Korea aut Seo Jae-Gu R&D Center, Cell Biotech, Co., Ltd, 134 Gaegok-Ri Wolgot-Myeon, 415-872, Gimpo-Si, Gyeonggi-Do, Korea aut Chung Myung-Jun R&D Center, Cell Biotech, Co., Ltd, 134 Gaegok-Ri Wolgot-Myeon, 415-872, Gimpo-Si, Gyeonggi-Do, Korea aut Yum Do-Young R&D Center, Cell Biotech, Co., Ltd, 134 Gaegok-Ri Wolgot-Myeon, 415-872, Gimpo-Si, Gyeonggi-Do, Korea aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/17(2015-09-01), 7089-7099 0175-7598 99:17<7089 2015 99 253 https://doi.org/10.1007/s00253-015-6455-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6455-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kim Min-Soo R&D Center, Cell Biotech, Co., Ltd, 134 Gaegok-Ri Wolgot-Myeon, 415-872, Gimpo-Si, Gyeonggi-Do, Korea aut NATIONALLICENCE 700 1- Kim Jin-Eung R&D Center, Cell Biotech, Co., Ltd, 134 Gaegok-Ri Wolgot-Myeon, 415-872, Gimpo-Si, Gyeonggi-Do, Korea aut NATIONALLICENCE 700 1- Yoon Yeo-Sang R&D Center, Cell Biotech, Co., Ltd, 134 Gaegok-Ri Wolgot-Myeon, 415-872, Gimpo-Si, Gyeonggi-Do, Korea aut NATIONALLICENCE 700 1- Kim Tai R&D Center, Cell Biotech, Co., Ltd, 134 Gaegok-Ri Wolgot-Myeon, 415-872, Gimpo-Si, Gyeonggi-Do, Korea aut NATIONALLICENCE 700 1- Seo Jae-Gu R&D Center, Cell Biotech, Co., Ltd, 134 Gaegok-Ri Wolgot-Myeon, 415-872, Gimpo-Si, Gyeonggi-Do, Korea aut NATIONALLICENCE 700 1- Chung Myung-Jun R&D Center, Cell Biotech, Co., Ltd, 134 Gaegok-Ri Wolgot-Myeon, 415-872, Gimpo-Si, Gyeonggi-Do, Korea aut NATIONALLICENCE 700 1- Yum Do-Young R&D Center, Cell Biotech, Co., Ltd, 134 Gaegok-Ri Wolgot-Myeon, 415-872, Gimpo-Si, Gyeonggi-Do, Korea aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/17(2015-09-01), 7089-7099 0175-7598 99:17<7089 2015 99 253