caa a22 4500 605503133 CHVBK 20210128100609.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00253-015-6488-2 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6488-2 pH regulates ammonia-oxidizing bacteria and archaea in paddy soils in Southern China [Elektronische Daten] [Hu Li, Bo-Sen Weng, Fu-Yi Huang, Jian-Qiang Su, Xiao-Ru Yang] Ammonia-oxidizing archaea (AOA) and bacteria (AOB) play important roles in nitrogen cycling. However, the effects of environmental factors on the activity, abundance, and diversity of AOA and AOB and the relative contributions of these two groups to nitrification in paddy soils are not well explained. In this study, potential nitrification activity (PNA), abundance, and diversity of amoA genes from 12 paddy soils in Southern China were determined by potential nitrification assay, quantitative PCR, and cloning. The results showed that PNA was highly variable between paddy soils, ranging from 4.05 ± 0.21 to 9.81 ± 1.09mg NOx-Nkg−1 dry soilday−1, and no significant correlation with soil parameters was found. The abundance of AOA was predominant over AOB, indicating that AOA may be the major members in aerobic ammonia oxidation in these paddy soils. Community compositions of AOA and AOB were highly variable among samples, but the variations were best explained by pH. AOA sequences were affiliated to the Nitrosopumilus cluster and Nitrososphaera cluster, and AOB were classified into the lineages of Nitrosospira and Nitrosomonas, with Nitrosospira being predominant over Nitrosomonas, accounting for 83.6% of the AOB community. Moreover, the majority of Nitrosomonas was determined in neutral soils. Canonical correspondence analysis (CCA) analysis further demonstrated that AOA and AOB community structures were significantly affected by pH, soil total organic carbon, total nitrogen, and C/N ratio, suggesting that these factors exert strong effects on the distribution of AOB and AOA in paddy soils in Southern China. In conclusion, our results imply that soil pH was a key explanatory variable for both AOA and AOB community structure and nitrification activity. Springer-Verlag Berlin Heidelberg, 2015 Paddy soil nationallicence Potential nitrification activity nationallicence Ammonia-oxidizing bacteria nationallicence Ammonia-oxidizing archaea nationallicence Southern China nationallicence Li Hu Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, 361021, Xiamen, China aut Weng Bo-Sen Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, 361021, Xiamen, China aut Huang Fu-Yi Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, 361021, Xiamen, China aut Su Jian-Qiang Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, 361021, Xiamen, China aut Yang Xiao-Ru Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, 361021, Xiamen, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/14(2015-07-01), 6113-6123 0175-7598 99:14<6113 2015 99 253 https://doi.org/10.1007/s00253-015-6488-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6488-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Hu Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, 361021, Xiamen, China aut NATIONALLICENCE 700 1- Weng Bo-Sen Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, 361021, Xiamen, China aut NATIONALLICENCE 700 1- Huang Fu-Yi Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, 361021, Xiamen, China aut NATIONALLICENCE 700 1- Su Jian-Qiang Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, 361021, Xiamen, China aut NATIONALLICENCE 700 1- Yang Xiao-Ru Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, 361021, Xiamen, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/14(2015-07-01), 6113-6123 0175-7598 99:14<6113 2015 99 253