caa a22 4500 605503141 CHVBK 20210128100609.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00253-015-6547-8 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6547-8 Functional gene-based discovery of phenazines from the actinobacteria associated with marine sponges in the South China Sea [Elektronische Daten] [Valliappan Karuppiah, Yingxin Li, Wei Sun, Guofang Feng, Zhiyong Li] Phenazines represent a large group of nitrogen-containing heterocyclic compounds produced by the diverse group of bacteria including actinobacteria. In this study, a total of 197 actinobacterial strains were isolated from seven different marine sponge species in the South China Sea using five different culture media. Eighty-seven morphologically different actinobacterial strains were selected and grouped into 13 genera, including Actinoalloteichus, Kocuria, Micrococcus, Micromonospora, Mycobacterium, Nocardiopsis, Prauserella, Rhodococcus, Saccharopolyspora, Salinispora, Serinicoccus, and Streptomyces by the phylogenetic analysis of 16S rRNA gene. Based on the screening of phzE genes, ten strains, including five Streptomyces, two Nocardiopsis, one Salinispora, one Micrococcus, and one Serinicoccus were found to be potential for phenazine production. The level of phzE gene expression was highly expressed in Nocardiopsis sp. 13-33-15, 13-12-13, and Serinicoccus sp. 13-12-4 on the fifth day of fermentation. Finally, 1,6-dihydroxy phenazine (1) from Nocardiopsis sp. 13-33-15 and 13-12-13, and 1,6-dimethoxy phenazine (2) from Nocardiopsis sp. 13-33-15 were isolated and identified successfully based on ESI-MS and NMR analysis. The compounds 1 and 2 showed antibacterial activity against Bacillus mycoides SJ14, Staphylococcus aureus SJ51, Escherichia coli SJ42, and Micrococcus luteus SJ47. This study suggests that the integrated approach of gene screening and chemical analysis is an effective strategy to find the target compounds and lays the basis for the production of phenazine from the sponge-associated actinobacteria. Springer-Verlag Berlin Heidelberg, 2015 Actinobacteria nationallicence Sponges nationallicence Diversity nationallicence Phenazine nationallicence Karuppiah Valliappan Marine Biotechnology Laboratory, State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 200240, Shanghai, People's Republic of China aut Li Yingxin Marine Biotechnology Laboratory, State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 200240, Shanghai, People's Republic of China aut Sun Wei Marine Biotechnology Laboratory, State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 200240, Shanghai, People's Republic of China aut Feng Guofang Marine Biotechnology Laboratory, State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 200240, Shanghai, People's Republic of China aut Li Zhiyong Marine Biotechnology Laboratory, State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 200240, Shanghai, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/14(2015-07-01), 5939-5950 0175-7598 99:14<5939 2015 99 253 https://doi.org/10.1007/s00253-015-6547-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6547-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Karuppiah Valliappan Marine Biotechnology Laboratory, State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 200240, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Li Yingxin Marine Biotechnology Laboratory, State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 200240, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Sun Wei Marine Biotechnology Laboratory, State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 200240, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Feng Guofang Marine Biotechnology Laboratory, State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 200240, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Li Zhiyong Marine Biotechnology Laboratory, State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 200240, Shanghai, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/14(2015-07-01), 5939-5950 0175-7598 99:14<5939 2015 99 253