caa a22 4500 60550315X CHVBK 20210128100609.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00253-015-6544-y doi (NATIONALLICENCE)springer-10.1007/s00253-015-6544-y Functional analysis of Trichoderma reesei CKIIα2, a catalytic subunit of casein kinase II [Elektronische Daten] [Mingyu Wang, Hui Yang, Meiling Zhang, Kuimei Liu, Hanbin Wang, Yi Luo, Xu Fang] Trichoderma reesei is the most important industrial cellulase-producing filamentous fungus. Although its molecular physiology has been investigated, the signal transduction pathways are not fully understood. In particular, the role of casein kinase II (CKII) is not yet clear. In this work, we carried out functional investigations on a catalytic subunit of CKII, CKIIα2. Comparison of the phenotypic features of T. reesei parent and Δck2α2 strains showed significant changes following ck2α2 disruption. T. reesei Δck2α2 form significantly smaller mycelial pellets in glucose-containing liquid minimum media, have shorter and fewer branch hyphae, produce smaller amounts of chitinases, produce more spores, show more robust growth on glucose-containing agar plates, and consume glucose at a significantly higher rate. Suggestions can be made that CKIIα2 governs chitinase expression, and the disruption of ck2α2 results in lower levels of chitinase production, leading to a weaker cell wall disruption capability, further resulting in weaker hyphal branching, which eventually leads to smaller mycelial pellets in liquid media. Further conclusions can be made that CKIIα2 is involved in repression of sporulation and glucose metabolism, which is consistent with the proposal that CKIIα2 represses global metabolism. These observations make the deletion of ck2α2 a potentially beneficial genetic disruption for T. reesei during industrial applications, as smaller mycelial pellets, more spores and more robust glucose metabolism are all desired traits for industrial fermentation. This work reports novel unique functions of a CKII catalytic subunit and is also the first genetic and physiological investigation on CKII in T. reesei. Springer-Verlag Berlin Heidelberg, 2015 Trichoderma reesei nationallicence Casein kinase II nationallicence Sporulation nationallicence Mycelial pellet nationallicence Glucose metabolism nationallicence Cellulase nationallicence Wang Mingyu State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, 250100, Jinan, China aut Yang Hui State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, 250100, Jinan, China aut Zhang Meiling State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, 250100, Jinan, China aut Liu Kuimei State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, 250100, Jinan, China aut Wang Hanbin School of Physics, Shandong University, 250100, Jinan, China aut Luo Yi School of Physics, Shandong University, 250100, Jinan, China aut Fang Xu State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, 250100, Jinan, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/14(2015-07-01), 5929-5938 0175-7598 99:14<5929 2015 99 253 https://doi.org/10.1007/s00253-015-6544-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6544-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Mingyu State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, 250100, Jinan, China aut NATIONALLICENCE 700 1- Yang Hui State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, 250100, Jinan, China aut NATIONALLICENCE 700 1- Zhang Meiling State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, 250100, Jinan, China aut NATIONALLICENCE 700 1- Liu Kuimei State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, 250100, Jinan, China aut NATIONALLICENCE 700 1- Wang Hanbin School of Physics, Shandong University, 250100, Jinan, China aut NATIONALLICENCE 700 1- Luo Yi School of Physics, Shandong University, 250100, Jinan, China aut NATIONALLICENCE 700 1- Fang Xu State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, 250100, Jinan, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/14(2015-07-01), 5929-5938 0175-7598 99:14<5929 2015 99 253