caa a22 4500 605503192 CHVBK 20210128100609.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00253-015-6628-8 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6628-8 A three-step method for analysing bacterial biofilm formation under continuous medium flow [Elektronische Daten] [Karolin Schmutzler, Andreas Schmid, Katja Buehler] For the investigation and comparison of microbial biofilms, a variety of analytical methods have been established, all focusing on different growth stages and application areas of biofilms. In this study, a novel quantitative assay for analysing biofilm maturation under the influence of continuous flow conditions was developed using the interesting biocatalyst Pseudomonas taiwanensis VLB120. In contrast to other tubular-based assay systems, this novel assay format delivers three readouts using a single setup in a total assay time of 40h. It combines morphotype analysis of biofilm colonies with the direct quantification of biofilm biomass and pellicle formation on an air/liquid interphase. Applying the Tube-Assay, the impact of the second messenger cyclic diguanylate on biofilm formation of P. taiwanensis VLB120 was investigated. To this end, 41 deletions of genes encoding for protein homologues to diguanylate cyclase and phosphodiesterase were generated in the genome of P. taiwanensis VLB120. Subsequently, the biofilm formation of the resulting mutants was analysed using the Tube-Assay. In more than 60% of the mutants, a significantly altered biofilm formation as compared to the parent strain was detected. Furthermore, the potential of the proposed Tube-Assay was validated by investigating the biofilms of several other bacterial species. Springer-Verlag Berlin Heidelberg, 2015 Pseudomonas taiwanensis VLB120 nationallicence Tubular biofilm nationallicence Biofilm dry weight nationallicence Pellicle nationallicence Single species biofilm nationallicence Cyclic diguanylate nationallicence Schmutzler Karolin Laboratory of Chemical Biotechnology, Department of Biochemical and Chemical Engineering, TU Dortmund University, Emil-Figge-Strasse 66, 44227, Dortmund, Germany aut Schmid Andreas Department of Solar Materials, Helmholtz-Centre for Environmental Research, Permoserstrasse 15, 04318, Leipzig, Germany aut Buehler Katja Laboratory of Chemical Biotechnology, Department of Biochemical and Chemical Engineering, TU Dortmund University, Emil-Figge-Strasse 66, 44227, Dortmund, Germany aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/14(2015-07-01), 6035-6047 0175-7598 99:14<6035 2015 99 253 https://doi.org/10.1007/s00253-015-6628-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6628-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schmutzler Karolin Laboratory of Chemical Biotechnology, Department of Biochemical and Chemical Engineering, TU Dortmund University, Emil-Figge-Strasse 66, 44227, Dortmund, Germany aut NATIONALLICENCE 700 1- Schmid Andreas Department of Solar Materials, Helmholtz-Centre for Environmental Research, Permoserstrasse 15, 04318, Leipzig, Germany aut NATIONALLICENCE 700 1- Buehler Katja Laboratory of Chemical Biotechnology, Department of Biochemical and Chemical Engineering, TU Dortmund University, Emil-Figge-Strasse 66, 44227, Dortmund, Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/14(2015-07-01), 6035-6047 0175-7598 99:14<6035 2015 99 253