caa a22 4500 605503206 CHVBK 20210128100609.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00253-015-6575-4 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6575-4 Selective formation of mannosyl- l -arabitol lipid by Pseudozyma tsukubaensis JCM16987 [Elektronische Daten] [Tomotake Morita, Tokuma Fukuoka, Ayana Kosaka, Tomohiro Imura, Hideki Sakai, Masahiko Abe, Dai Kitamoto] To develop a structural homolog of mannosylerythritol lipids (MELs), Pseudozyma tsukubaensis JCM16987 (known to be a specific producer of the diastereomer type of mono-acetylated MEL (MEL-B)) was cultivated in medium containing 4% (w/v) olive oil as the primary carbon source and 4% l-arabitol as the supplemental sugar alcohol. Based on thin-layer chromatography (TLC), the glycolipid extract showed two major spots corresponding to MEL-B and an unknown glycolipid (GL1). Based on high-performance liquid chromatography after acid hydrolysis, GL1 from the l-arabitol culture showed two primary peaks identical to mannose and arabitol using the sugar analysis column, and one peak identical to l-arabitol was detected using the chiral resolution column. Based on NMR analysis, GL1 was identified as mono-acetylated mannosyl-l-arabitol lipid (MLAL-B) consisting of mannose, with l-arabitol as the sugar moiety. The observed critical micelle concentration (CMC) and surface tension at the CMC (γCMC) of MLAL-B were 1.2 × 10−5M and 32.8mN/m, which were significantly higher than MEL-B (CMC = 3.1 × 10−6M and γcmc = 26.1mN/m). Furthermore, based on a water-penetration scan, MLAL-B efficiently formed lamellar phase (Lα) and myelins at a broad concentration range. Thus, the present glycolipid showed higher hydrophilicity and/or water solubility and increased our understanding of environmentally advanced biosurfactants. Springer-Verlag Berlin Heidelberg, 2015 Mannosylarabitol lipid nationallicence l -Arabitol nationallicence Glycolipid nationallicence Yeast nationallicence Pseudozyma nationallicence Morita Tomotake Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, Higashi 1-1-1, 305-8565, Tsukuba, Ibaraki, Japan aut Fukuoka Tokuma Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, Higashi 1-1-1, 305-8565, Tsukuba, Ibaraki, Japan aut Kosaka Ayana Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, Higashi 1-1-1, 305-8565, Tsukuba, Ibaraki, Japan aut Imura Tomohiro Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, Higashi 1-1-1, 305-8565, Tsukuba, Ibaraki, Japan aut Sakai Hideki Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, 278-8510, Noda, Chiba, Japan aut Abe Masahiko Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, 278-8510, Noda, Chiba, Japan aut Kitamoto Dai Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, Higashi 1-1-1, 305-8565, Tsukuba, Ibaraki, Japan aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/14(2015-07-01), 5833-5841 0175-7598 99:14<5833 2015 99 253 https://doi.org/10.1007/s00253-015-6575-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6575-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Morita Tomotake Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, Higashi 1-1-1, 305-8565, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Fukuoka Tokuma Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, Higashi 1-1-1, 305-8565, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Kosaka Ayana Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, Higashi 1-1-1, 305-8565, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Imura Tomohiro Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, Higashi 1-1-1, 305-8565, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Sakai Hideki Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, 278-8510, Noda, Chiba, Japan aut NATIONALLICENCE 700 1- Abe Masahiko Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, 278-8510, Noda, Chiba, Japan aut NATIONALLICENCE 700 1- Kitamoto Dai Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, Higashi 1-1-1, 305-8565, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/14(2015-07-01), 5833-5841 0175-7598 99:14<5833 2015 99 253