Comprehensive mapping infection-enhancing epitopes of dengue pr protein using polyclonal antibody against prM
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6538-9 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6538-9 | ||
| 245 | 0 | 0 | |a Comprehensive mapping infection-enhancing epitopes of dengue pr protein using polyclonal antibody against prM |h [Elektronische Daten] |c [Yayan Luo, Xiaolan Guo, Huijun Yan, Danyun Fang, Gucheng Zeng, Junmei Zhou, Lifang Jiang] |
| 520 | 3 | |a Dengue vaccine development is considered a global public health priority, but the antibody-dependent enhancement (ADE) issues have critically restricted vaccine development. Recent findings have demonstrated that pre-membrane (prM) protein was involved in dengue virus (DENV) infection enhancement. Although the importance of prM antibodies have been well characterized, only a few epitopes in DENV prM protein have ever been identified. In this study, we screened five potential linear epitopes located at positions pr1 (1-16aa), pr3 (13-28aa), pr4 (19-34aa), pr9 (49-64aa), and pr10 (55-70aa) in pr protein using peptide scanning and comprehensive bioinformatics analysis. Then, we found that only pr4 (19-34aa) could elicit high-titer antibodies in Balb/c mice, and this epitope could react with sera from DENV2-infected patients, suggesting that specific antibodies against epitope peptide pr4 were elicited in both DENV-infected mice and human. In addition, our data demonstrated that anti-pr4 sera showed limited neutralizing activity but significant ADE activity toward standard DENV serotypes and imDENV. Hence, it seems responsible to hypothesize that anti-pr4 serum was infection-enhancing antibody and pr4 was infection-enhancing epitope. In conclusion, we characterized a novel infection-enhancing epitope on dengue pr protein, a finding that may provide new insight into the pathogenesis of DENV infection and contribute to dengue vaccine design. | |
| 540 | |a The Author(s), 2015 | ||
| 690 | 7 | |a Dengue virus |2 nationallicence | |
| 690 | 7 | |a pr protein |2 nationallicence | |
| 690 | 7 | |a Epitope |2 nationallicence | |
| 690 | 7 | |a Synthetic peptides |2 nationallicence | |
| 690 | 7 | |a Antibody-dependent enhancement |2 nationallicence | |
| 700 | 1 | |a Luo |D Yayan |u Guangzhou Brain Hospital (Guangzhou Huiai hospital, the affiliated hospital of Guangzhou Medical University), 510370, Guanghzou, China |4 aut | |
| 700 | 1 | |a Guo |D Xiaolan |u Key Laboratory for Tropic Diseases Control, Ministry of Education of China, Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, China |4 aut | |
| 700 | 1 | |a Yan |D Huijun |u Key Laboratory for Tropic Diseases Control, Ministry of Education of China, Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, China |4 aut | |
| 700 | 1 | |a Fang |D Danyun |u Key Laboratory for Tropic Diseases Control, Ministry of Education of China, Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, China |4 aut | |
| 700 | 1 | |a Zeng |D Gucheng |u Key Laboratory for Tropic Diseases Control, Ministry of Education of China, Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, China |4 aut | |
| 700 | 1 | |a Zhou |D Junmei |u Key Laboratory for Tropic Diseases Control, Ministry of Education of China, Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, China |4 aut | |
| 700 | 1 | |a Jiang |D Lifang |u Key Laboratory for Tropic Diseases Control, Ministry of Education of China, Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/14(2015-07-01), 5917-5927 |x 0175-7598 |q 99:14<5917 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6538-9 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6538-9 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Luo |D Yayan |u Guangzhou Brain Hospital (Guangzhou Huiai hospital, the affiliated hospital of Guangzhou Medical University), 510370, Guanghzou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Guo |D Xiaolan |u Key Laboratory for Tropic Diseases Control, Ministry of Education of China, Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yan |D Huijun |u Key Laboratory for Tropic Diseases Control, Ministry of Education of China, Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Fang |D Danyun |u Key Laboratory for Tropic Diseases Control, Ministry of Education of China, Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zeng |D Gucheng |u Key Laboratory for Tropic Diseases Control, Ministry of Education of China, Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhou |D Junmei |u Key Laboratory for Tropic Diseases Control, Ministry of Education of China, Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Jiang |D Lifang |u Key Laboratory for Tropic Diseases Control, Ministry of Education of China, Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/14(2015-07-01), 5917-5927 |x 0175-7598 |q 99:14<5917 |1 2015 |2 99 |o 253 | ||