caa a22 4500 605503214 CHVBK 20210128100609.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00253-015-6538-9 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6538-9 Comprehensive mapping infection-enhancing epitopes of dengue pr protein using polyclonal antibody against prM [Elektronische Daten] [Yayan Luo, Xiaolan Guo, Huijun Yan, Danyun Fang, Gucheng Zeng, Junmei Zhou, Lifang Jiang] Dengue vaccine development is considered a global public health priority, but the antibody-dependent enhancement (ADE) issues have critically restricted vaccine development. Recent findings have demonstrated that pre-membrane (prM) protein was involved in dengue virus (DENV) infection enhancement. Although the importance of prM antibodies have been well characterized, only a few epitopes in DENV prM protein have ever been identified. In this study, we screened five potential linear epitopes located at positions pr1 (1-16aa), pr3 (13-28aa), pr4 (19-34aa), pr9 (49-64aa), and pr10 (55-70aa) in pr protein using peptide scanning and comprehensive bioinformatics analysis. Then, we found that only pr4 (19-34aa) could elicit high-titer antibodies in Balb/c mice, and this epitope could react with sera from DENV2-infected patients, suggesting that specific antibodies against epitope peptide pr4 were elicited in both DENV-infected mice and human. In addition, our data demonstrated that anti-pr4 sera showed limited neutralizing activity but significant ADE activity toward standard DENV serotypes and imDENV. Hence, it seems responsible to hypothesize that anti-pr4 serum was infection-enhancing antibody and pr4 was infection-enhancing epitope. In conclusion, we characterized a novel infection-enhancing epitope on dengue pr protein, a finding that may provide new insight into the pathogenesis of DENV infection and contribute to dengue vaccine design. The Author(s), 2015 Dengue virus nationallicence pr protein nationallicence Epitope nationallicence Synthetic peptides nationallicence Antibody-dependent enhancement nationallicence Luo Yayan Guangzhou Brain Hospital (Guangzhou Huiai hospital, the affiliated hospital of Guangzhou Medical University), 510370, Guanghzou, China aut Guo Xiaolan Key Laboratory for Tropic Diseases Control, Ministry of Education of China, Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, China aut Yan Huijun Key Laboratory for Tropic Diseases Control, Ministry of Education of China, Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, China aut Fang Danyun Key Laboratory for Tropic Diseases Control, Ministry of Education of China, Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, China aut Zeng Gucheng Key Laboratory for Tropic Diseases Control, Ministry of Education of China, Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, China aut Zhou Junmei Key Laboratory for Tropic Diseases Control, Ministry of Education of China, Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, China aut Jiang Lifang Key Laboratory for Tropic Diseases Control, Ministry of Education of China, Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/14(2015-07-01), 5917-5927 0175-7598 99:14<5917 2015 99 253 https://doi.org/10.1007/s00253-015-6538-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6538-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Luo Yayan Guangzhou Brain Hospital (Guangzhou Huiai hospital, the affiliated hospital of Guangzhou Medical University), 510370, Guanghzou, China aut NATIONALLICENCE 700 1- Guo Xiaolan Key Laboratory for Tropic Diseases Control, Ministry of Education of China, Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, China aut NATIONALLICENCE 700 1- Yan Huijun Key Laboratory for Tropic Diseases Control, Ministry of Education of China, Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, China aut NATIONALLICENCE 700 1- Fang Danyun Key Laboratory for Tropic Diseases Control, Ministry of Education of China, Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, China aut NATIONALLICENCE 700 1- Zeng Gucheng Key Laboratory for Tropic Diseases Control, Ministry of Education of China, Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, China aut NATIONALLICENCE 700 1- Zhou Junmei Key Laboratory for Tropic Diseases Control, Ministry of Education of China, Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, China aut NATIONALLICENCE 700 1- Jiang Lifang Key Laboratory for Tropic Diseases Control, Ministry of Education of China, Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/14(2015-07-01), 5917-5927 0175-7598 99:14<5917 2015 99 253