caa a22 4500 605503222 CHVBK 20210128100609.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00253-015-6492-6 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6492-6 In vitro and in vivo antibacterial activity of environmental bacteriophages against Pseudomonas aeruginosa strains from cystic fibrosis patients [Elektronische Daten] [Tomasz Olszak, Paulina Zarnowiec, Wieslaw Kaca, Katarzyna Danis-Wlodarczyk, Daria Augustyniak, Pavel Drevinek, Anthony de Soyza, Siobhán McClean, Zuzanna Drulis-Kawa] The goal of the study was to determine the relationship between in vitro/in vivo efficacy of environmental Pseudomonas phages and certain phenotypical properties of Pseudomonas aeruginosa (PA) strains. We studied the diversity between particular isolates and determined phage sensitivity in vitro and in vivo in the Galleria mellonella insect model. Twenty-eight lytic bacteriophages specific for PA were tested against 121 CF PA isolates including 29 mucoid PA strains. Most strains from cystic fibrosis (CF) patients were lysed by at least three phages (93.6%), but completely insensitive strains were also present (6.4%). Two phages PA5oct and KT28 exhibited high rates of lytic potency on 55-68% of PA strains (72-86% of mucoid isolates). We further explored phage activity against six PA strains (CF and non-CF) in vitro, comparing clonal differences in phage susceptibility with bacterial properties such as the ability to form biofilms, mucosity, twitching motility, and biochemical profiles. We observed the relationship between variation in phage susceptibility and Fourier transform infrared spectroscopy (FTIR) analysis in the spectra window of carbohydrates. The protective efficacy of two selected phages against PA PAO1 and 0038 infection was confirmed in vivo in G. mellonella larvae. Generally, the wax moth model results confirmed the data from in vitro assays, but in massive infection of CF isolates, the application of lytic phages probably led to the release of toxic compound causing an increase in larvae mortality. We assumed that apart of in vitro phage activity testing, a simple and convenient wax moth larvae model should be applied for the evaluation of in vivo effectiveness of particular phage preparations. The Author(s), 2015 Myoviridae bacteriophages nationallicence Phage treatment nationallicence Pseudomonas aeruginosa nationallicence Cystic fibrosis nationallicence Galleria mellonella model nationallicence Olszak Tomasz Institute of Genetics and Microbiology, University of Wroclaw, Przybyszewskiego 63/77, 51-148, Wroclaw, Poland aut Zarnowiec Paulina The Jan Kochanowski University, Swietokrzyska 15, 25-406, Kielce, Poland aut Kaca Wieslaw The Jan Kochanowski University, Swietokrzyska 15, 25-406, Kielce, Poland aut Danis-Wlodarczyk Katarzyna Institute of Genetics and Microbiology, University of Wroclaw, Przybyszewskiego 63/77, 51-148, Wroclaw, Poland aut Augustyniak Daria Institute of Genetics and Microbiology, University of Wroclaw, Przybyszewskiego 63/77, 51-148, Wroclaw, Poland aut Drevinek Pavel Department of Medical Microbiology, University Hospital Motol and 2nd Faculty of Medicine, Charles University, 150 06, Prague, Czech Republic aut de Soyza Anthony Institute of Cellular Medicine, Medical School, Newcastle University, Framlington Place, NE2 4HH, Newcastle upon Tyne, UK aut McClean Siobhán Centre of Microbial Host Interactions, Institute of Technology Tallaght, Tallaght, Dublin 24, Ireland aut Drulis-Kawa Zuzanna Institute of Genetics and Microbiology, University of Wroclaw, Przybyszewskiego 63/77, 51-148, Wroclaw, Poland aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/14(2015-07-01), 6021-6033 0175-7598 99:14<6021 2015 99 253 https://doi.org/10.1007/s00253-015-6492-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6492-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Olszak Tomasz Institute of Genetics and Microbiology, University of Wroclaw, Przybyszewskiego 63/77, 51-148, Wroclaw, Poland aut NATIONALLICENCE 700 1- Zarnowiec Paulina The Jan Kochanowski University, Swietokrzyska 15, 25-406, Kielce, Poland aut NATIONALLICENCE 700 1- Kaca Wieslaw The Jan Kochanowski University, Swietokrzyska 15, 25-406, Kielce, Poland aut NATIONALLICENCE 700 1- Danis-Wlodarczyk Katarzyna Institute of Genetics and Microbiology, University of Wroclaw, Przybyszewskiego 63/77, 51-148, Wroclaw, Poland aut NATIONALLICENCE 700 1- Augustyniak Daria Institute of Genetics and Microbiology, University of Wroclaw, Przybyszewskiego 63/77, 51-148, Wroclaw, Poland aut NATIONALLICENCE 700 1- Drevinek Pavel Department of Medical Microbiology, University Hospital Motol and 2nd Faculty of Medicine, Charles University, 150 06, Prague, Czech Republic aut NATIONALLICENCE 700 1- de Soyza Anthony Institute of Cellular Medicine, Medical School, Newcastle University, Framlington Place, NE2 4HH, Newcastle upon Tyne, UK aut NATIONALLICENCE 700 1- McClean Siobhán Centre of Microbial Host Interactions, Institute of Technology Tallaght, Tallaght, Dublin 24, Ireland aut NATIONALLICENCE 700 1- Drulis-Kawa Zuzanna Institute of Genetics and Microbiology, University of Wroclaw, Przybyszewskiego 63/77, 51-148, Wroclaw, Poland aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/14(2015-07-01), 6021-6033 0175-7598 99:14<6021 2015 99 253